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.肽链延长因子eEF1A-1和eEF1A-2在小鼠神经元中的表达特征
引用本文:宋兴辉,王娜,顾黛君,傅强,尹苗,潘杰..肽链延长因子eEF1A-1和eEF1A-2在小鼠神经元中的表达特征[J].中国生物化学与分子生物学报,2006,22(2):124-128.
作者姓名:宋兴辉  王娜  顾黛君  傅强  尹苗  潘杰
作者单位:1. 浙江大学生命科学学院,杭州,310058
2. 山东师范大学生命科学学院,济南,250014
摘    要:研究2种肽链延长因子(eEF1A-1,eEF1A-2)在不同发育阶段的小鼠神经元中的表达特征,探 讨其调控机制.应用Western印迹和组织免疫荧光技术分析两蛋白质在不同基因型小鼠(n =10)神经细胞中的表达水平和分布.结果表明,在胚胎期和幼龄期的野生型小鼠神经元胞 质中,eEF1A-1呈高水平表达并随发育而下降,于出生后26 d时停止表达;而eEF1A-2蛋白于出生后7 d开始表达并呈上调趋势,出生后20 d时达到最高水平,其后一直保持稳定表达,2种肽链延长因子在野生型小鼠神经元中的表达随发育而呈相反变化.eEF1A_2基因突变小鼠无eEF1A-2蛋白,eEF1A_1蛋白的表达模式与野生型小鼠基本类似,但出生后26 d时仍有微量表达.2种肽链延长因子在野生型小鼠发育阶段的表达水平变化受内在机制调控,不直接受各自表达水平的影响;eEF1A_2蛋白与神经元生理功能的维持有密切关系.

关 键 词:肽链延长因子  神经元  基因突变  免疫荧光  
收稿时间:2005-06-13
修稿时间:2005年6月13日

Characteristic of Expression Patternof Peptide Elongation Factors eEF1A-1 and eEF1A-2 in Mouse Neuron
SONG Xing-Hui,WANG Na,GU Dai-Jun,FU Qiang,YIN Miao,PAN Jie.Characteristic of Expression Patternof Peptide Elongation Factors eEF1A-1 and eEF1A-2 in Mouse Neuron[J].Chinese Journal of Biochemistry and Molecular Biology,2006,22(2):124-128.
Authors:SONG Xing-Hui  WANG Na  GU Dai-Jun  FU Qiang  YIN Miao  PAN Jie
Institution: College of Life Sciences, Zhejiang University, Hangzhou310058,China;College of Life Sciences, Shandong Normal University, Jinan250014, China)
Abstract:The expression patterns of two isoforms of peptide elongation factors,eEF1A_1 and eEF1A_2 were investigated in central nervous system during the fetal and postnatal mice.Both immunoblotting and laser confocal micrography analysis have found that eEF1A_2 protein was highly expressed in cytoplasma of neurons in wild type mice from fetuses to neonatal day 1,thereafter slowly declining and becoming undetectable after 26 days of age.Interestingly,in eEF1A_2 mutant mice,however,eEF1A_1 expression was last longer than that of wild type control.In contrast,eEF1A_2 was being detected from 7 day old mice and then gradually increased,reaching the highest level at age of 20 days,keeping stable for life span.Further study showed that as the postnatal mice development eEF1A_2 alowly substituted its isoform eEF1A_1,and finally replaced this factor completely by 26 days of age.The reverse profile of two isoform proteins in neurons during fetal and postnatal mice development suggested that there are unidentified switch control mechanism for eEF1A_1 and eEF1A_2 expression,elucidation of which is in progress.
Keywords:peptide elongation factors  neuron  gene mutant  immunofluorescence  
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