猪SFRP4基因的表达规律及sp600125对前体脂肪细胞分化的影响

分泌型卷曲相关蛋白（SFRP4, secreted frizzled-related protein 4）是Wnt信号通路可溶解的调控子.本研究通过高通量测序（Solexa）技术、实时定量PCR（RT-qPCR）对瘦肉型和脂肪型猪不同生长阶段脂肪组织中SFRP4表达规律进行研究；用western免疫印迹及RT-PCR技术对脂肪细胞分化过程中SFRP4蛋白表达和mRNA表达进行检测；用JNK信号通路特异性抑制剂sp600125处理猪原代前体脂肪细胞，研究抑制JNK信号通路对猪前体脂肪分化以及SFRP4 mRNA和蛋白表达的影响.结果显示，SFRP4 在脂肪型猪脂肪组织表达量显著高于瘦肉型猪(P<0.01)；不同组织检测结果发现，SFRP4广泛表达于各个组织，并高表达于脂肪组织；前体脂肪细胞向成熟脂肪细胞分化过程中SFRP4表达量逐渐升高；sp600125促进了前体脂肪细胞分化，引起了 PPARγ、FABP4 、ATGL、Perilipin的显著升高(P<0.01)，而SFRP4的表达被显著抑制.本研究为调控脂肪细胞分化关键基因的筛选提供新的理论参考.

Expression of Porcine SFRP4 and Its Effect on Differentiation of Preadipocytes via Inhibitor SP600125

Secreted frizzled-related protein 4(SFRP4) was the regulator of Wnt signaling pathway.The expression of SFRP4 in fat tissues of different genotype pigs and in different growth periods were explored by high-throughout sequencing(Solexa) and real-time quantity PCR(RT-qPCR).The effect on differentiation of preadipocyte and the expression level of SFRP4 were explored by inhibitor SP600125 in JNK signaling pathway.The results showed that expression level of SFRP4 was higher in fat tissues of obese-type than that in the lean-type.Expression level of SFRP4 in the porcine fat tissue and adipocyte was influenced by ages,and was higher in fat,but lower in other tissues.SP600125 promoted the adiocytes to differentiate and significantly increased expression levels of PPARγ,FABP4,ATGL,Perilipin,but the SFRP4 expression level was inhibited significantly.This research provides a new reference for screen the key genes in adipocyte differentiation.