大熊猫、小熊猫和黑熊血红蛋白的组份分离、结晶及氨基酸组成分析

 聚丙烯酰胺凝胶电泳和羧甲基纤维素柱层析都表明大熊猫(Ailuropodmelanoleuca)、小熊猫(Ailurus fulgens)和黑熊(Selenarctos thibetanus)的血红蛋白各有两个组份。在近似的条件下得到了它们的晶体。三种动物血红蛋白的氨基酸组成相似,而且N-末端氨基酸都是Val。有待进一步测定全部氨基酸顺序,以揭示它们之间的异同和亲缘关系。

ISOLATION OF COMPONENTS, CRYSTALLIZATION AND AMINO ACID COMPOSITION OF HEMOGLOBIN FROM GIANT PANDA, LESSER PANDA AND BLACK BEAR

Both polyacrylamide gel electrophoresis and chromatography on CM-cellulose-22 showed that there are two components in hemoglobin isolated from giant panda (Ailuropoda melanoleuca), lesser panda(Ailurus fulgens)and black bear (Selenarctos thibetanus) respectively. Their red crystals were obtained in different forms. Amino acid composition analysis showed that the three hemoglobins are similar. Like the a and β chain of most mammal hemoglobins their N-terminal residue is Valine. More work should be done before we can draw any conclusion about whether the giant panda is closer to lesser panda or black bear.