长链非编码RNA RP11-214F16.8 促进乳腺癌细胞增殖

长链非编码RNAs (long non-coding RNAs, lncRNAs)在乳腺癌发生发展中的作用备受瞩目。本研究通过大数据分析发现，lncRNA RP11-214F16.8在乳腺癌组织中的表达量显著高于正常组织，且其表达量与乳腺癌患者预后负相关。因此，本文采用qRT-PCR技术，在收集的临床样本中验证RP11-214F16.8的表达，证实其在乳腺癌组织中相对表达量为5.65±0.72，其在癌旁组织中表达量为2.63±0.35，且RP11-214F16.8的表达量与乳腺癌瘤体大小和临床TNM分期相关。此外发现，RP11-214F16.8在乳腺癌细胞中的表达量高于正常乳腺上皮细胞。在乳腺癌MCF-7细胞中，过表达RP11-214F16.8后，MCF-7细胞的增殖能力显著增强。而在MDA-MB-231细胞中，敲低RP11-214F16.8的表达，获得相反的结果。进一步研究发现，RP11-214F16.8可上调细胞周期蛋白 D3、CDK4的表达，而下调p18蛋白表达量，进而加速细胞增殖。总之，RP11-214F16.8在乳腺癌中高表达，且促进乳腺癌细胞增殖，进而推动乳腺癌进程。这一研究发现，将为乳腺癌发生发展的网络机制研究提供新的理论依据。

Long Non-coding RNA RP11-214F16.8 Enhances Proliferation of Breast Cancer Cells

Long non-coding RNAs (lncRNAs) were highlighted in the progression of breast cancer. In this study, it was found that RP11-214F16.8 was significantly enhanced in breast cancer tissues in data set analysis by GEPIA software, comparing with that in normal. Moreover, RP11-214F16.8 was negatively correlated with the prognosis of breast cancer patients. Furthermore, qRT PCR assay was performed to determine the expression levels of RP11-214F16.8 in breast cancer and normal tissues collected at our hospital. The results showed that RP11-214F16.8 was highly expressed in breast cancer tissues (5.65±0.72) than that in normal tissues (2.63±0.35). RP11-214F16.8 expression was positively correlated with tumor size and TNM stage of breast cancer. Similarly, it was demonstrated that RP11-214F16.8 was evidently accelerated in breast cancer cells, comparing with that in mammary MCF-10A epithelial cells. Restoring RP11-214F16.8 enhanced proliferation of MCF-7 cells, while silencing RP11-214F16.8 reduced MDA-MB-231 cells proliferation. RP11-214F16.8 could upregulate cyclin D3 and CDK4 protein expression while downregulated p18 protein expression. To sum up, RP11-214F16.8 was highly expressed in breast cancer and enhanced proliferation of breast cancer cells. This investigation can provide new insight into the molecular mechanisms of breast cancer progression.