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MCP-1通过p38和ERK/MAPK途径在人单核细胞中诱导Stat3丝氨酸磷酸化
引用本文:谭运年,金亚平.MCP-1通过p38和ERK/MAPK途径在人单核细胞中诱导Stat3丝氨酸磷酸化[J].中国生物化学与分子生物学报,2009,25(5):448-453.
作者姓名:谭运年  金亚平
作者单位:(浙江大学医学院附属第二医院生化室,杭州310009)
摘    要:

关 键 词:单核细胞趋化因子1  信号转导激活转录因子3  丝氨酸磷酸化  细胞外信号调节激酶  p38  
收稿时间:2008-12-20

MCP-1 Inducing Stat3 Serine Phosphorylation via p38 and ERK Pathways in Human Monocytes
TAN Yun-Nian,Jin Ya-Ping.MCP-1 Inducing Stat3 Serine Phosphorylation via p38 and ERK Pathways in Human Monocytes[J].Chinese Journal of Biochemistry and Molecular Biology,2009,25(5):448-453.
Authors:TAN Yun-Nian  Jin Ya-Ping
Institution:(Section of Clinical Biochemistry, Department of Laboratory Medicine, Second Affiliated Hospital, Zhejiang University, Hangzhou 310009,China)
Abstract:Monocyte chemoattactant protein-1(MCP-1) plays a crucial role in the recruitment of monocytes associated with several inflammatory disease and malignancies. In order to investigate Stat3 phosphorylation induced by MCP-1 in human monocytes, we used anti phospho STAT3 (Tyr 705) and (Ser 727) antibodies in Western blot analysis to identify that 727 serine phosphorylation of Stat3 was induced in monocytes only. Serine phosphorylation of Stat3 induced by MCP-1 was in time and dose dependent fashion. Moreover, in the MAPK pathway involved in Stat3 serine phosphorylation investigation, we observed that MCP-1 could trigger p38 and ERK (extracellular signal-regulated kinase) pathways but not JNK pathway in time dependent fashion. A few inhibitors of p38 and ERK activation used to block the serine phosphorylation of Stat3 induced by MCP-1 identified that both p38 and ERK pathways involved in serine phosphorylation of Stat3 in human monocytes. Taken together, MCP-1 can only trigger Stat3 serine phosphorylation and not tyrosine phosphorylation via p38 and ERK pathways in human monocytes.
Keywords:monocyte chemoattactant protein-1  Stat3  serine phosphorylation  ERK  p38
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