| 巴豆毒蛋白的分离、结晶及其性质研究 |
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| 引用本文： | 陈明晃,潘克桢.巴豆毒蛋白的分离、结晶及其性质研究[J].中国生物化学与分子生物学报,1993,9(1):104-108. |
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| 作者姓名： | 陈明晃 潘克桢 |
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| 作者单位： | 中国科学院福建物质结构研究所，中国科学院福建物质结构研究所 福州 350002，福州 350002 |
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| 基金项目： | 国家自然科学基金，福建省自然科学基金 |
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| 摘 要： | 巴豆种子用PBS抽提,多次低溫离心去除大量油脂,经硫酸铵沉淀,从Sophadex G-75柱分离出巴豆毒蛋白Ⅰ和Ⅱ(CrotinⅠ,Ⅱ)SDS-PAGB测得其分子量为40kD和15kD;等电点分别为8.0和6.7,并测定了它们的氨基酸组成。巴豆毒蛋白Ⅱ用汽相扩散悬滴法获得结晶,蛙卵试验表明它具有较强的抑制蛋白质合成的活性。
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| 关 键 词： | 巴豆毒蛋白 核糖体失活蛋白 分离 结晶 |
| 收稿时间： | 1993-02-20 |
Isolation,Characterization and Crystallization of Crotins Toxin from the Seeds of Croton tiglium L. |
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| Chen,Ming-huang Pan,Ke-zhen.Isolation,Characterization and Crystallization of Crotins Toxin from the Seeds of Croton tiglium L.[J].Chinese Journal of Biochemistry and Molecular Biology,1993,9(1):104-108. |
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| Authors: | Chen Ming-huang Pan Ke-zhen |
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| Institution: | (Fujian Institute of Research on the Structure of Matter, Academia Sinica, Fuzhou 350002 |
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| Abstract: | Two toxins, Crotin Ⅰ, Ⅱ were isolated from the seeds of Croton tiglium by extraction with PBS, precipitation with ammonium sulfate followed by gel filtration through Sephadex G-75. The molecular weights estimated by SDS-PAGE were 40kD and 15kD and the isoelectric point estimated to be 8.0 and 6.7, respectively. The acute LD_(50) evaluated at 72h was 0.45 mg/mouse for Crotin Ⅰ and 2.23mg/mouse for Crotin Ⅱ. Crotin Ⅱ strongly inhibits protein synthesis in eukaryotic ribosome by oocyte of the toad test and belongs to Ribosome-inactivating Proteins. Crotin Ⅱ was crystallized by hanging droplet method. |
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| Keywords: | Crotin Ribosome-inactivating proteins Isolation crystallization |
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