| 高比活力大肠杆菌磷酸转乙酰酶的纯化 |
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| 引用本文： | 张光兴,张元亮,张增弟.高比活力大肠杆菌磷酸转乙酰酶的纯化[J].中国生物化学与分子生物学报,1988,4(6):496-501. |
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| 作者姓名： | 张光兴 张元亮 张增弟 |
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| 作者单位： | 天津商学院食品工程系，河北大学生物系，河北大学生物系 保定，保定 |
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| 摘 要： | 从国内大肠杆菌菌株1.505提取磷酸转乙酰酶(PTA),经数步纯化荻得了高比活力酶制品。该酶制品比粗酶纯化了61倍,比活力达1040单位/mg,经聚丙烯酰胺凝胶电泳检查,仅有两条蛋白质区带。用经过纯化的酶测定辅酶A,其结果6.75单位/10微升酶液在光径1cm时,233nm的吸收值△E_(PTA)<0.009。
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| 关 键 词： | 大肠杆菌 磷酸转乙酰酶 辅酶A |
| 收稿时间： | 1988-12-20 |
PURIFIED PROCESS OF PHOSPHOTRANSACETYLASE FROM Escherichia Coll WITH HIGH SPECIFIC ACTVITY |
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| Zhang,Guang-xing Zhang,Yuan-Liang Zhang,Zeng-di.PURIFIED PROCESS OF PHOSPHOTRANSACETYLASE FROM Escherichia Coll WITH HIGH SPECIFIC ACTVITY[J].Chinese Journal of Biochemistry and Molecular Biology,1988,4(6):496-501. |
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| Authors: | Zhang Guang-xing Zhang Yuan-Liang Zhang Zeng-di |
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| Institution: | (Department of Food Engineering (Department of Biology,Tianjin Commerical College, Tianjin) Hebei University, Baoding |
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| Abstract: | A process of purification of PTA from E. Coli 1,505 with high specific activity has been reported in this paper. This PTA has been purified 61 times over the crude extraction with 5.7% recovery and specific activity 1040U/mg. The purified PTA preparation has only two clear bands in polyacrylamide gel electro?phoresis. Coenzyme A has been assessed with purified PTA: 6.75U/10μl PTA solution at light-path 1cm wavelenght 233nm, the optical density difference, △EPTA is less than 0.009. |
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| Keywords: | Escherichia Coli Pbosphotransacetylase Coenzyme A |
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