| 放射性配体结合分析法测定鸡视网膜多巴胺D1受体 |
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| 引用本文： | 肖林,贺师鹏,陈瑞英,郭静秋,黄天贵.放射性配体结合分析法测定鸡视网膜多巴胺D1受体[J].中国生物化学与分子生物学报,1997,13(2):169-172. |
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| 作者姓名： | 肖林 贺师鹏 陈瑞英 郭静秋 黄天贵 |
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| 作者单位： | 北京医科大学第一医院小儿眼科!北京，100083(肖林，陈瑞英，郭静秋)，北京医科大学第一医院生物物理系!北京，100083(贺师鹏，黄天贵) |
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| 基金项目： | 卫生部科研基金!C082 |
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| 摘 要： | 用放射性配体3H]SCH23390建立鸡视网膜多巴胺D1受体的放射性配体结合分析法，并测定不同试验组鸡视网膜多巴胺D1受体的Bmax值有显著性差异，KD值无明显差异．同时探讨了干，湿滤膜片对放射性测定结果的影响，以及影响试验结果的其它因素．实验表明湿滤膜片测量效果优于干滤膜片，膜受体即使贮存在-20℃条件下亦只能一周，否则影响受体的活性．
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| 关 键 词： | 鸡实验性近视 多巴胺D1受体 放射性配体结合分析 湿滤膜片法 |
| 收稿时间： | 1997-04-20 |
The Radioligand Binding Assay of Dopamine D1 Receptor in Chicken in vitro |
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| Xiao Lin,He Shi-Peng,Chen Rui-Ying,Guo Jing-Qiu,Huang Tian-Gui.The Radioligand Binding Assay of Dopamine D1 Receptor in Chicken in vitro[J].Chinese Journal of Biochemistry and Molecular Biology,1997,13(2):169-172. |
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| Authors: | Xiao Lin He Shi-Peng Chen Rui-Ying Guo Jing-Qiu Huang Tian-Gui |
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| Institution: | (Department of Pediatric Ophthol.First Hospital.Department of Biophysics, Beijing Medical University, Beijing 100083 |
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| Abstract: | The Dopamine D1 receptor in chicken retina in vitro was studied with 3H]SCH23390 (D1 selective antagonists). The radioligand binding assay of dopamine receptor in chicken retina was established; and the effect of dry and wet filters and other factors on the measurement of scintillation counters was studied. The results indicated that it is better to use wet filters for assay than to use'dry filter. The receptor in membrane should be kept at - 20℃ for only a week. The difference of Bmax was obvious in different groups but the KD was not obvious |
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| Keywords: | Radioligand-binding-assay Chicken-retina Dopamine-D1-receptor Filters |
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