鸡红细胞膜囊泡及其蛋白质组成

叙述了一种以囊泡形式制取鸡红细胞膜的方法。该囊泡呈杆菌状,大小约2.02×0.47μm。其SDS-PAGE谱虽类似于人红细胞膜影泡,但:鸡囊泡无甘油醛-3-磷酸脱氢酶;α和β收缩蛋白呈宽间隔双体带,带3蛋白由两种变体,B3.1和B3.2所组成;含有人红细胞膜没有的Goblin、中间丝和来自核膜的组蛋白。用胰蛋白酶原位消化鸡囊泡时,带3消失并在Mr=55和36kD处显两新带,后者并不存在同条件下人红细胞原位酶解谱中,说明两种带3在膜中构象并不相同。

Protein Composition of Chicken Erythrocyte Membrane Vesicles

A method for preparing chicken erythrocyte membranes in the form of small vesicles has been described.The vesicles are bacilliform about 2.02*********x0.47*********um.Although the SDS-PAGE pattern of the vesicles is similar to that of human erythrocyte ghosts, there are still some differences in the two species.The chicken vesicles are totally devoid of glyceraldehyde-3-phosphate dehydrogenase.**********a- and *********b-spectrin of the vesicles can be resolved into a pattern of loosely spaced doublet on 7.5% SDS-PAGE.The vesicles contain goblin,intermediate filament proteins and histons derived from nuclear membrane.Band 3 protein of the vesicles is composed of two variants, B3.1 and B3.2, and they appear as a doublet on the underloaded gel.When the alkali-stripped vesicles were treated with trypsin in situ, the band 3 disappear and two new bands with Mr=55 and 36kD are seen while the band with 36 kD is not present in trypsintreated of human erythrocyte ghosts under the same condition.Thus, the two band 3 species differ in the sites of trypsin cleavage in the membrane, suggesting that the in conformation in the bilayer may be different.