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非水溶剂中Rhizopus arrhizus脂肪酶催化合成三种酯的最佳条件(英文)
引用本文:杨本宏,吴克,刘斌,郑敏,蔡敬民,潘仁瑞.非水溶剂中Rhizopus arrhizus脂肪酶催化合成三种酯的最佳条件(英文)[J].中国生物化学与分子生物学报,2003,19(5):572-575.
作者姓名:杨本宏  吴克  刘斌  郑敏  蔡敬民  潘仁瑞
作者单位:合肥联合大学化学与生物工程系,合肥,230022
基金项目:安徽省自然科学基金资助 (No.0 10 43 10 2 )~~
摘    要:以少根根霉 (Rhizopusarrhizus)脂肪酶为催化剂 ,有机溶剂为反应介质 ,合成了 3种短链脂肪酸酯 .研究了反应温度、溶剂、底物浓度、底物摩尔比、吸水剂用量等因素对酯化反应的影响 .确定了3种酯的最佳合成条件 :(1)己酸乙酯 :反应温度为 4 0℃ ,环己烷为溶剂 ,0 2 5mol L底物浓度 ,酸醇摩尔比为 1∶1 2 ;(2 )乙酸异丙酯 :5 0℃ ,环己烷为溶剂 ,0 15mol L底物浓度 ,摩尔比为 1∶1;(3)乙酸异戊酯 :5 0℃ ,异辛烷为溶剂 ,0 2 0mol L底物浓度 ,摩尔比为 1∶1.三种酯合成时均需 0 12 5g ml的0 5nm分子筛为吸水剂 ,在 8h后 ,合成酯转化率达到 97%~ 99% .

关 键 词:脂肪酶  酯化反应  己酸乙酯  乙酸异丙酯  乙酸异戊酯  最佳条件  
收稿时间:2003-10-20

Rhizopus arrhizus Lipase Catalyzed Syntheses of Three Esters in Nonaqueous Solvents
Abstract.Rhizopus arrhizus Lipase Catalyzed Syntheses of Three Esters in Nonaqueous Solvents[J].Chinese Journal of Biochemistry and Molecular Biology,2003,19(5):572-575.
Authors:Abstract
Institution:(Department of Chemical and Biological Engineering, Hefei Union University, Hefei 230022, China
Abstract:The Rhizopus arrhizus lipase catalyzed syntheses of three short-chain esters in organic solvents were studied. The optimum conditions for each esterification were determined as follows: (1)ethyl caproate: synthesized in cyclohexane at 40℃ with 0.25 mol/L substrate and 1∶1.2 acid-alcohol molar ratio; (2)isopropyl acetate: reacted in cyclohexane at 50℃ with 0.15 mol/L substrate and 1∶1 molar ratio; (3)isoamyl acetate: in isooctane at 50℃ with 0.20 mol/L substrate, and 1∶1 molar ratio. 0.125 g per ml of 0.5 nm molecular sieve was required as adsorbent for water in the reactions. Under the optimal conditions, the yields reached 97%-99% after incubation for 8 hours.
Keywords:lipase  esterification  ethyl caproate  isopropyl acetate  isoamyl acetate    optimization
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