蛇床子素通过促进胃癌细胞N87凋亡和细胞周期阻滞而抑制细胞增殖

蛇床子素是从伞形科植物蛇床中提取的一类具有生物活性的化合物。研究显示,蛇床子素对多种肿瘤细胞具有抑制作用,然而尚未有研究揭示其对胃癌N87细胞的抗肿瘤活性。本文研究了蛇床子素在体外和荷瘤小鼠体内对胃癌N87细胞的抗肿瘤效应,并进一步利用流式细胞术、TUNEL试验及Western印迹检测分析其对细胞周期及细胞凋亡的影响,以探索其作用机制。研究结果表明,蛇床子素有效地抑制了体外培养的N87细胞生长,并呈浓度依赖效应。本文还建立了N87的荷瘤小鼠模型。结果显示,无论是在低剂量(50 mg/kg)或高剂量(100 mg/kg)情况下,蛇床子素均显示了有效的肿瘤生长抑制效果。流式细胞术及Western印迹的结果表明,蛇床子素诱导N87细胞阻滞在G_2/M期。通过流式细胞术、TUNEL测试及Western印迹结果证明,蛇床子素通过激活胱天蛋白酶-3依赖的凋亡通路,最终导致了N87细胞凋亡的发生。综上所述,本研究显示,蛇床子素在胃癌N87细胞中通过促进细胞凋亡而发挥其抗肿瘤活性,这将为其应用于胃癌的临床治疗提供理论参考。

Osthole Inhibits Cell Proliferation by Promoting Apoptosis and Cell Cycle Arrest in Gastric Cancer Cell Line N87

Osthole is a natural active coumarin, which was first derived from Cnidium monnieri (L.) Cusson. Recent studies revealed that osthole possesses potent anti-tumor activity in several types of cancers. However, the anti-tumor effects of osthole against N87 gastric cancer cells have not been reported. We studied the anti-tumor effects of osthole on N87 cells in vitro and in vivo, and further explored the mechanism. The effects of osthole on cell cycle arrest and apoptosis were examined by flow cytometry and Western blotting assays. Results showed that osthole was effective in inhibiting the growth of N87 cells in a dose-dependent manner. Notably, we found that osthole also exert its anti-tumor effects on N87 tumor xenografts at both low and high doses. Furthermore, we investigated its mechanism using flow cytometry, Western blotting and TUNEL assays. Results indicated that osthole caused cell cycle arrest in the G2/M phase and finally resulted in caspase-3 dependent apoptosis in N87 cells. To conclude, these results revealed the anti-tumor activity of osthole on N87 gastric cancer cells and uncovered the mechanism of action, suggesting the potential of osthole in gastric cancer treatment in the future.