| 骨骼肌M蛋白抗血清的制备及正常人骨骼肌M蛋白的免疫电镜定位 |
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| 引用本文： | 屈竹青,卢鼎厚.骨骼肌M蛋白抗血清的制备及正常人骨骼肌M蛋白的免疫电镜定位[J].中国生物化学与分子生物学报,1994,10(2):140-145. |
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| 作者姓名： | 屈竹青 卢鼎厚 |
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| 作者单位： | 北京体育学院运动生理教研室 |
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| 摘 要： | 分别制备了兔抗人Ｍ蛋白（Ｂ成分）抗血清和兔抗人Ｃ成分［１］抗血清。用蛋白Ａ－胶体金作标记物，对经ＬｏｗｉｃｒｙｌＫ４Ｍ低温包埋的人骨骼肌超薄切片中Ｍ蛋白和分子量１４００００的Ｃ成分进行免疫电镜定位。发现Ｍ蛋白分布于整个Ｍ线，而Ｃ成分虽然也集中于Ｍ线以内，但主要分布于Ｍ线内的边缘区域。
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| 关 键 词： | M蛋白 C成分 抗血清 免疫金标记 |
| 收稿时间： | 1994-04-20 |
Preparation of Rabbit Anti-protein Antiserum and the Immunoelectron Microscopic Localization of M-protein of Human Skeletal Muscle |
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| Qu,Zhuqing,Lu,Ding-hou.Preparation of Rabbit Anti-protein Antiserum and the Immunoelectron Microscopic Localization of M-protein of Human Skeletal Muscle[J].Chinese Journal of Biochemistry and Molecular Biology,1994,10(2):140-145. |
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| Authors: | Qu Zhuqing Lu Ding-hou |
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| Institution: | (Section of Exercise Physiology,Beijing Institute of Physical Education,Beijing 100084 |
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| Abstract: | The rabbit anti-M-protein and anti-C component antisera were prepared seperately. Using the protein-A-gold complex as the label, the M-protein and MW 140000 component C were localized by immuno electron microscopy in the ultrathin sections of the human skeletal muscle embeded in the low temperature medium Lowicryl K4M. The M-protein is well distributed in the whole M-line, but the component C is localized mainly at the margin within the M-line. |
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| Keywords: | M-protein C component Antiserum Immunogold labelling |
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