| 自由基与软骨基质胶原蛋白类型改变 |
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| 引用本文： | 张法浩,程天蓉,常淑云,许善锦,王夔.自由基与软骨基质胶原蛋白类型改变[J].中国生物化学与分子生物学报,1994,10(2):155-158. |
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| 作者姓名： | 张法浩 程天蓉 常淑云 许善锦 王夔 |
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| 作者单位： | 北京医科大学药学院 |
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| 基金项目： | 国家“八五”科技攻关课题 |
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| 摘 要： | 本文利用ＳＤＳ－聚丙烯酰胺凝胶电泳方法，定量研究了体外培养的软骨细胞和软骨组织基质中Ⅱ型胶原蛋白的含量。结果表明氧自由基（·Ｏ－２和·ＯＨ）和具有自由基性质的物质（黄腐酸，镰刀菌毒素）可使软骨细胞合成，分泌异常的非Ⅱ型的胶原蛋白，同时，硒化合物可明显地抑制此种效应。
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| 关 键 词： | 自由基 软骨细胞 胶原蛋白 |
| 收稿时间： | 1994-04-20 |
Effects of Free Radicals on the Collagen Type Change in Cartilage Matrix |
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| Zhang,Fa-hao,Cheng,Tian-rong,Chang,Shu-yun,Xu,Shan-jin,Wang,Kui.Effects of Free Radicals on the Collagen Type Change in Cartilage Matrix[J].Chinese Journal of Biochemistry and Molecular Biology,1994,10(2):155-158. |
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| Authors: | Zhang Fa-hao Cheng Tian-rong Chang Shu-yun Xu Shan-jin Wang Kui |
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| Institution: | (School of Pharmaceutical Sciences,Beijing Medical University,Beijing 100083 |
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| Abstract: | In this paper, a method of determining the percentage of type Ⅱ collagen in extracellular matrix of cultured chondrocytes or caritilage tissue in terms of the spectrophotometric densities of specific cyangen bromide peptide bonds on SDS-polyacrylamide gels has been used. The results show that the free radical generating systems, including Fe(Ⅱ)-EDTA,Xanthine Oxidase-Xanthine. Fulvic acid and Fusarium toxin, can cause chondrocytes to synthesize and secrete mainly type Ⅰ collagen instead of the normal type Ⅱ collagen. In addition, The collagen type change can be inhibited by Se(Ⅳ) compounds. |
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| Keywords: | Free radical Collagen Chondrocyte |
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