| 不同年龄小白鼠全脑细胞质氨酰tRNA合成酶活力的比较 |
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| 引用本文： | 张承圭,吕慧梅,王新光,郑集,王启松,商金宝.不同年龄小白鼠全脑细胞质氨酰tRNA合成酶活力的比较[J].中国生物化学与分子生物学报,1985,1(4):75-78. |
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| 作者姓名： | 张承圭 吕慧梅 王新光 郑集 王启松 商金宝 |
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| 作者单位： | 南京大学生物化学系
(张承圭，吕慧梅，郑集)，南京大学同位素室
(王新光)，科学院上海生物化学研究所
(王启松)，科学院上海生物化学研究所(商金宝) |
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| 摘 要： | 从不同年龄(20天,30天,1年)的小白鼠全脑制得细胞质混合氨酰tRNA合成酶。用异源体系(即用酵母tRNA和小白鼠全脑氨酰tRNA合成酶)测定了氨酰tRNA合成酶分别载运~3H标记的Asp、Gly、Glu、Lys和Ala的活力。结果表明除未检出tRNA~(Glu)的合成酶活力外,对其余四种氨基酸都有明显的活力,特别是年龄20天小白鼠的氨酰tRNA合成酶对~3H-Gly具有高达35％的载运活力。对~3H-Gly、~3H-Lys和~3H-Ala的载运活力有随增龄而下降的趋势,但对~3H-Asp的载运活力则随年龄增长而增高。
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| 关 键 词： | 年龄 鼠脑 氨酰tRNA合成酶 |
| 收稿时间： | 1985-08-20 |
Age-related Changes in the Aminoacyl-tRNA Sythetase Activities of Whole Mouse Brain |
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| Zhang Cheng-gui,Lu Hui-mei,Wang Xing-guang,Cheng,L,T.Nanjing UmiversityWang Qi-song,Shang Jin-baoShavghai Iniljluls of Bivchemistry,Academia. Sinica.Age-related Changes in the Aminoacyl-tRNA Sythetase Activities of Whole Mouse Brain[J].Chinese Journal of Biochemistry and Molecular Biology,1985,1(4):75-78. |
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| Authors: | Zhang Cheng-gui Lu Hui-mei Wang Xing-guang Cheng L TNanjing UmiversityWang Qi-song Shang Jin-baoShavghai Iniljluls of Bivchemistry Academia Sinica |
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| Abstract: | Results in this paper indicate that the aminoacyl-tRNA synthetases, prepared from brain tissues of mouse at different age have different charging activities to tRNAs of aspartic acid, glycine, lysine and alanine but no detectable- activity to charge glutamic acid tRNA in a heterologous assay system containing yeast tRNA and mouse aminoacyl-tRNA synthetase.Aminoacyl-tRNA synthetase activities related to glycine lysine and alanine are tending to decrease with increase in age, but the age-related charge in activtty is just reversed in the case of aspartic acid tRNA synthetase. |
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| Keywords: | Age Mouse brain Aminoacyl-tRNA syntnetase |
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