首页 | 本学科首页   官方微博 | 高级检索  
   检索      

灵芝(Ganoderma lucidum)漆酶基因的克隆及其序列分析
引用本文:张银波,江木兰,胡小加,张桂敏,马立新.灵芝(Ganoderma lucidum)漆酶基因的克隆及其序列分析[J].中国生物化学与分子生物学报,2005,21(5):699-703.
作者姓名:张银波  江木兰  胡小加  张桂敏  马立新
作者单位:1. 湖北大学生命科学学院分子微生物与基因工程研究室,武汉,430062;中国农业科学院油料作物研究所,农业部油料作物遗传改良重点开放实验室,武汉,430062
2. 中国农业科学院油料作物研究所,农业部油料作物遗传改良重点开放实验室,武汉,430062
3. 湖北大学生命科学学院分子微生物与基因工程研究室,武汉,430062
基金项目:国家“863”计划(No.2001AA214161,No.2002AA227011)资助~~
摘    要:漆酶(laccase EC1·10·3·2)是一种含Cu的多酚氧化酶,自从1883年日本学者吉田首次从漆树汁液中发现以来,漆酶特别是真菌漆酶一直是生物学、化学和环境科学等领域十分活跃的研究热点,在纸浆的生物漂白1,2],有毒污染物的降解3~5]等方面有较大的应用价值.根据其来源主要分为漆树漆酶和真菌漆酶两大类,由一个结构相似的基因家族所编码,目前,至少有40个以上的真菌漆酶基因被克隆和测序6~9],最近也有从细菌中克隆到漆酶基因的报道10~12].我国幅员辽阔,具有十分丰富的真菌资源,但是我国对漆酶的研究与发达国家相比还十分落后,对于漆酶基因资…

收稿时间:2005-10-20
修稿时间:2004年10月28

Cloning and Sequence Analysis of a Laccase Gene from Ganoderma lucidum
ZHANG Yin-Bo,JIANG Mu-Lan,HU Xiao-Jia,ZHANG Gui-Min,MA Li-Xin.Cloning and Sequence Analysis of a Laccase Gene from Ganoderma lucidum[J].Chinese Journal of Biochemistry and Molecular Biology,2005,21(5):699-703.
Authors:ZHANG Yin-Bo  JIANG Mu-Lan  HU Xiao-Jia  ZHANG Gui-Min  MA Li-Xin
Institution:(1) Molecular Microbiology Laboratory, Hubei University, Wuhan 430062, China; 2) Oil Crops Research Institute, CAAS and Key Laboratory of Genetics Improvement for Oil Crops, Ministry of Agriculture, Wuhan 430062, China
Abstract:The oxidizing enzyme laccase produced by many fungi is generally considered to be active in the biodegradation of lignin, a major plant cell wall component highly resistant to microbial attack. Using degenerate primers and molecular techniques such as genome-walking and RT-PCR, a laccase gene and its corresponding cDNA were cloned from Ganoderma lucidum. The coding region of the genomic laccase sequence, which is preceded by the eukaryotic promoter elements TATA and CAAT, spans more than 2107 bp. The corresponding laccase cDNA was identical to the genomic sequence except for 9 introns that were 51-78 bp long. Sequence analysis indicated that the G. lucidum lccGl product has 10 potential N-glycosylation sites (Asn-Xxx-Ser/Thr) and four conserved fungi laccase amino acids signature sequences. The G. lucidum laccase sequence is most similar to the sequence of the laccase from Flammulina velutipes (similarity 73%).
Keywords:laccase gene  genome-walking  sequence analysis
本文献已被 CNKI 万方数据 等数据库收录!
点击此处可从《中国生物化学与分子生物学报》浏览原始摘要信息
点击此处可从《中国生物化学与分子生物学报》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号