聚己内酯静电纺丝纳米纤维支架用于小鼠诱导多能干细胞培养

干细胞联合生物支架材料体外构建功能性组织与器官，成为当前组织再生研究的重要策略，而探求具有良好生物相容性的支架材料是其关键.本研究采用扫描电镜、噻唑蓝（MTT）法、荧光显微染色等方法检测小鼠诱导多能干细胞（murine induced pluripotent stem cells, miPSCs）在聚己内酯（poly ε-caprolactone, PCL）静电纺丝纳米纤维支架上的粘附、增殖等生物学特性，探究聚己内酯纳米纤维支架与miPSCs的生物相容性. 结果显示,miPSC在PCL纳米纤维支架上具有良好粘附性并呈集落样生长，其增殖能力及干性标记物（Oct4-GFP+）的表达均不亚于标准对照组；扫描电镜显示,miPSC在PCL纳米纤维支架材料上呈现出绒毛状突起的表面结构.上述结果表明,PCL纳米纤维支架可促进miPSCs的粘附、自我增殖以及干性维持，两者具有良好的生物相容性，为下一步联合生物支架材料与干细胞构建功能性组织奠定了基础.

Electrospun Poly(ε-caprolactone) Nanofibrous Scaffolds for Mouse Induced Pluripotent Stem Cell Cultivation

In vitro engineering of functional tissue and organ with stem cells and biomaterial scaffold become a significant strategy for tissue regeneration at present, whereas exploring scaffolds with good biocompatibility is critical. To evaluate the biocompatibility of murine induced pluripotent stem cell (miPSCs) on the poly ε-caprolactone (PCL) nanofibrous scaffold, in this study, the biological behavior of miPSCs on the PCL nanofibrous scaffold, such as adhesion and proliferation were detected using scanning electron microscopy, MTT assay and fluorescence staining. The results showed that miPSCs adhered well and grew in colony on the PCL nanofibrous scaffold, similar to Oct4-GFP+ miPSCs cultured with MEF feeder-layers in tissue culture plates. Moreover, the proliferation and pluripotency (expression of Oct4-GFP+) of miPSCs in scaffold group were as good as control group. Scanning electron micrographs revealed that the surface topography of miPSCs grown on the PCL nanofibrous scaffold was characterized by a cilium-like structure. These results demonstrated that the electrospun PCL nanofibrous scaffold could promote the adhension, self-renewal and pluripotency maintenance of miPSCs, and had a good biocompatibility with Oct4-GFP+ miPSCs, which laid foundations for further research on functional tissue-engineering with stem cells and biomaterial scaffold.