| 臭鼩高重复顺序DNA的核苷酸顺序分析及其与树鼩TSr(Bgl Ⅱ)-1顺序的比较 |
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| 引用本文： | 袁建刚,熊伟军,林海玉,蔡良琬.臭鼩高重复顺序DNA的核苷酸顺序分析及其与树鼩TSr(Bgl Ⅱ)-1顺序的比较[J].中国生物化学与分子生物学报,1988,4(5):385-391. |
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| 作者姓名： | 袁建刚 熊伟军 林海玉 蔡良琬 |
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| 作者单位： | 中国医学科学院基础医学研究所 北京
(袁建刚，熊伟军，林海玉)，中国医学科学院基础医学研究所 北京(蔡良琬) |
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| 摘 要： | 将臭鼩DAN经过Bam H Ⅰ酶切得到的高重复顺序DNA最小片段重组到质粒pAT153上,转化后得到了含有臭鼩BMS(Bam H Ⅰ)-1高重复顺序DNA片段的克隆。再把此片段重组到M_(13)mp19噬菌体DNA上。用末端终止法测得全部苷酸顺序为495个碱基对。对臭鼬BMS(Bam H Ⅰ)-1片段的结构特点进行了分析,并和树鼩TSr(BglⅡ)-1高重复顺序DNA进行了比较。为确定树鼩在分类学上的地位,提供了一定的分子遗传学证据。
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| 关 键 词： | 臭鼩 DNA-片段 高重复顺序 分子克隆 序列分析 树鼩 |
| 收稿时间： | 1988-10-20 |
THE NUCLEOTIPE SEQUENCE OF BROWN MUSK SHREW'S HIGHLY REPEATED DNA AND COMPARISON WITH TSr (BglⅡ)-1 FRAGMENT OF TREE SHREW DNA |
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| Yuan,Jian-gang Xiong,Wei-jun Lin,Hai-yu Cai,Liang-wan.THE NUCLEOTIPE SEQUENCE OF BROWN MUSK SHREW'S HIGHLY REPEATED DNA AND COMPARISON WITH TSr (BglⅡ)-1 FRAGMENT OF TREE SHREW DNA[J].Chinese Journal of Biochemistry and Molecular Biology,1988,4(5):385-391. |
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| Authors: | Yuan Jian-gang Xiong Wei-jun Lin Hai-yu Cai Liang-wan |
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| Institution: | (Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing |
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| Abstract: | Highly repeated DNA fragment in genomic DNA of Brown Musk Shrow was separated by digestion with BamH I. The smallest fragment BMS(BamH I )-l was re-combined with the plasmid pAT153. After transformation into E. coli,the BMS(Bam HI) clone, was obtained. Then the cloned BMS (BamH J )-1 fragment was insered in M13mp19 DNA and it's nucleotide sequence was analysed. The whole nucleotide sequence of BMS (BamH I )-1 is 495bP. To determine the possible correlation between the highly repeated sequence of Brown Musk Shrow's DNA and that of Tree Shrow's DNA, the BMS (BamH I )-1 fragment was compared with the TSr (B-glⅡ)-1 fragment.The results showed that the BMS (BamH I )-1 is different from the TSr (BglⅡ)-1 in many aspects. |
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| Keywords: | Brown Musk Shrow Fragment of DNA Highly repeated sequence Molecular cloning Analysis of nucleotide sequence Tree shrow |
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