首页 | 本学科首页   官方微博 | 高级检索  
   检索      

血管再狭窄发生过程中MMP-2基因表达与胶原转换的动态变化
引用本文:周秀霞,温进坤,韩梅.血管再狭窄发生过程中MMP-2基因表达与胶原转换的动态变化[J].中国生物化学与分子生物学报,1999,15(6):997-1001.
作者姓名:周秀霞  温进坤  韩梅
作者单位:河北医科大学基础医学研究所生化研究室!石家庄050017,河北医科大学基础医学研究所生化研究室!石家庄050017,河北医科大学基础医学研究所生化研究室!石家庄050017
基金项目:国家自然科学基金,河北省自然科学基金
摘    要:建立主动脉内皮剥脱后血管再狭窄大鼠模型,用Northern 印迹分析、含明胶SDS-PAGE、羟脯氨酸含量测定及流式细胞术动态观察血管再狭窄发生过程中MMP-2基因表达与胶原转换及血管细胞增殖之间的关系.实验结果表明,血管内皮剥脱后,MMP-2基因表达被显著诱导,MMP-2活性及胶原转换明显增高,在第7 d,MMP-2表达活性及胶原合成与降解均达到峰值;第21 d,MMP-2表达恢复正常,胶原合成和降解仍维持在较高水平.流式细胞分析结果显示,血管内皮剥脱后进入增殖状态及发生凋亡的细胞显著增加,增殖细胞所占比例高于凋亡细胞,且两者均随内皮损伤后的时间延长而增加.

关 键 词:球囊损伤  血管再狭窄  基质金属蛋白酶-2  基因表达  胶原转换  细胞增殖  细胞凋亡  
收稿时间:1999-12-20

The Change of MMP-2 Gene Expression and Collagen Turnover during Vascular Restenosis Development
ZHOU Xiuxia,WEN Jinkun,HAN Mei.The Change of MMP-2 Gene Expression and Collagen Turnover during Vascular Restenosis Development[J].Chinese Journal of Biochemistry and Molecular Biology,1999,15(6):997-1001.
Authors:ZHOU Xiuxia  WEN Jinkun  HAN Mei
Institution:(Department of Biochemistry,Institute of Basic Medicine,Hebei Medical University,Shijiazhuang 050017
Abstract:A rat model of vascular restenosis was established by balloon injury.Using Northern blot analysis,SDS PAGE containing gelatin,hydroxyproline concentration determination and flow cytometry analysis,MMP 2 gene expression,collagen turnover and vascular cell proliferation activity were analyzed during vascular restenosis development.The results showed that MMP 2 gene expression was markedly induced by vascular de endothelialization,then MMP 2 activity and collagen turnover increased obviously.On day 7,MMP 2 gene expression,the collagen synthesis and degradation reached the peaks;On day 21,MMP 2 expression returned to basic level.Cell cycle analysis suggested that the number of proliferating and apoptotic cells increased remarkably during vascular restenosis development.With the extension of the time of endothelial injury,both the proliferating cells and apoptotic cells increased,but the proportion of the former was higher than that of the latter.
Keywords:Balloon injury  Vascular restenosis  MMP-2  Gene expression  Collagen turnover  Cell proliferation  Cell apoptosis
本文献已被 CNKI 等数据库收录!
点击此处可从《中国生物化学与分子生物学报》浏览原始摘要信息
点击此处可从《中国生物化学与分子生物学报》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号