| 玉米蛋白质二硫键异构酶（PDI）基因的特征和表达 |
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| 引用本文： | 刘颖慧 ,王秀堂 ,石云素,黄亚群,宋燕春 ,王天宇,黎 裕.玉米蛋白质二硫键异构酶（PDI）基因的特征和表达[J].中国生物化学与分子生物学报,2009,25(3):229-234. |
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| 作者姓名： | 刘颖慧 王秀堂 石云素 黄亚群 宋燕春 王天宇 黎 裕 |
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| 作者单位： | 1)中国农业科学研究院作物科学研究所，北京100081；2)河北农业大学农学院，河北保定071001；3)河北北方学院，河北张家口075000 |
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| 摘 要： | 蛋白质二硫键异构酶（PDI）对蛋白的折叠和二硫键的形成起重要的作用.此外，PDI还执行许多其他的生物功能，是1个多功能酶. 本文通过研究玉米中1个PDI基因的特征和表达，探讨它的功能作用. 玉米中的PDI基因编码513个氨基酸.同源分析表明,该基因和水稻、小麦的PDI基因聚为一类，有很高的蛋白相似性.蛋白结构分析表明,该基因具有明显的PDI基因的结构特点，包括硫氧还蛋白活性位点(CGHC)以及内质网定位信号(KDEL).Northern杂交分析显示,该基因在发育种子的表达量高，同时受干旱、冷、ABA和盐等逆境胁迫诱导表达.PDI与GFP融合表达研究基因的亚细胞定位，表明该基因定位在除细胞膜外的细胞质和细胞器上.
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| 关 键 词： | 蛋白质二硫键异构酶 玉米 基因表达 亚细胞定位 |
| 收稿时间： | 2008-7-10 |
| 修稿时间： | 2008-10-16 |
Expression and Characterization of a Protein Disulfide Isomerases in Maize (Zea Mays L.) |
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| LIU Ying-Hui ,WANG Xiu-Tang,SHI Yun-Su,HUANG Ya-Qun,SONG Yan-Chun,WANG Tian-Yu,LI Yu.Expression and Characterization of a Protein Disulfide Isomerases in Maize (Zea Mays L.)[J].Chinese Journal of Biochemistry and Molecular Biology,2009,25(3):229-234. |
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| Authors: | LIU Ying-Hui WANG Xiu-Tang SHI Yun-Su HUANG Ya-Qun SONG Yan-Chun WANG Tian-Yu LI Yu |
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| Institution: | 1)Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/ National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Beijing 100081, China;2)College of Agronomy, Hebei Agricultural University, Baoding 071001, China;3)Hebei North University, Zhangjiakou 075000, China |
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| Abstract: | Protein disulfide isomerases (PDIs) play an important roles in protein folding and redox signaling in plants. In this paper, we analyzed the characteristics and functions of a PDI from maize. The maize PDI gene included an open reading frame of 513 amino acids, which showed a significant conservation with PDIs from wheat and rice. All 3 PDIs have typical structures of two thioredoxin like active sites (CGHC) and an endoplasmic reticulum retention signal at its C-terminus (KDEL). Northern blotting showed that maize PDI had a higher expression level in developing seeds and could be strongly induced under dehydration, cold, salt and abscisic acid (ABA) stresses. The expression of PDI-GFP fusion protein revealed its localization in both nuclear and cytoplasm compartments. |
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| Keywords: | protein disulfide isomerase (PDI) maize gene expression subcellular localization |
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