猪IGF-I基因的一个遗传多态性及其遗传效应分析

采用PCR-SSCP方法对长白猪（87头）、大白猪（79头）和马身猪（102头）的胰岛素样生长因子-I（IGF-I）基因exon3和exon4分别进行单核苷酸多态性分析。发现exon3上有多态性，且存在3种基因型（AA、AB、BB）。统计结果表明，3种基因型在各品种中的分布不一致，多重比较差异极显著（P<0.01）。固定效应模型分析结果表明，背膘厚基因型间差异显著（P<0.05），而初生重、断奶重和6月龄重基因型间差异不显著（P>0.05）。最小二乘分析结果表明，BB基因型与其它2种基因型比较有较大的初生重，同AA和AB型比较差异极显著（P<0.01），3种基因型在初生重的大小排列顺序为AA

One Genetic Polymorphisms and Genetic Effects of IGF-I Gene in Pigs

Single nucleotide polymorphisms of 87 Landrace pigs, 78 Large White pigs and 102 Mashen pigs at exon3 and exon4 of IGF-I gene were tested by PCR-SSCP. One polymorphism was found at exon3, three genotypes exist (AA，AB，BB). Results of χ2 test showed that frequencies of genotypes in different breeds differ significantly from each other (P<0.01). Based on the fixed effect model, significant differences were found among IGF-I genotypes on backfat thickness, while no significant difference existed on birth weight, weaning weight and 6-month body weight; Using least square analysis, individuals of the BB genotype had significantly higher (P<0.01) birth weight than those of AA and AB genotypes, with the order being AA