[A_(15)Asn,A_(17)Pro,A_(21)Ala]-胰岛素的体内外生物活性

对胰岛素的研究使得许多生命科学中的重要问题被认识．然而对于胰岛素结构和功能关系，仍有许多不清楚的地方．在胰岛素Ａ链的Ｎ端和Ｃ端各有一个螺旋区：Ａ２－８和Ａ１２－１８．杨士珍［１］的研究表明：缺失Ａ１２－１８肽段的Ａ链类似物虽然能和天然Ｂ链重组，但其重组产物只保留了天然胰岛素的约６％的体内外生物活性．这说明Ａ１２－１８对胰岛素的生物活性十分重要．但是这一结果还不能说明是Ａ１２－１８螺旋还是Ａ１２－１８肽段对胰岛素的生物活性十分重要．根据Ｃｈｕｏ－Ｆａｓｍａｎ法，在含有Ａｓｎ和Ｐｒｏ的肽段中不能形成…

Biological Activities of [A_(15)Asn,A_(17) Pro,A_(21)Ala] Insulin

A 15 Asn,A 17 Pro,A 21 Ala] insulin was synthesized by the Fmoc solid phase synthetic method to study the biological significance of the helix in the C terminal region of A chain of insulin.In order to make this helix unable to form,Asn and Pro were utilized to substitute A 15 Gln and A 17 Glu,respectively,according to the Chuo Fasman method.The biological activity assays showed that this insulin analogue held only 10% of the biological activities of native insulin.The results suggest that the loss of the A12 18 helix in A chain leads to a remarkable decrease of biological activities of insulin,and this helix is significant to the three dimensional structure and biological functions of insulin.