着床期家兔子宫内膜糖蛋白的动态研究

本文以~3H-glucosamine(~3H-GlcNH_2)为前体,观察了着床期家兔子宫内膜糖蛋白的生物合成及分泌功能的变化,并利用Triton X-100提取膜蛋白,采用凝集素亲和电泳技术对三种不同性质糖蛋白进行了动态分析。结果表明,着床期家兔子宫内膜糖蛋白的合成与分泌功能均于妊娠第六天(D_6)明显增强,其中合成糖蛋白的变化以Triton溶解蛋白部分为主;井发现着床期WGA、RCA-Ⅰ、SBA结合蛋白均于着床前(D_6)明显增加,着床后(D_9)WGA结合蛋白略有降低,RCA-Ⅰ、SBA结合蛋白明显降低,接近未孕水平。着床期子宫内膜总体及不同种类糖蛋白的阶段特异性变化表明,在子宫内膜由对胚泡的非接受状态转变为接受态的过程中,阶段特异性糖蛋白可能具有重要作用。

Dynamic Research of Glycoprotein in Peri-implantation Stage Rabbit Endometrium

The function of glycoprotein secretion and synthesis in rabbit endometrium at peri-implantation stage was observed by radio-labelled sugar precursor (~3H-GlcNH_2) incorporation. The technique of lectin-affinity electrophoresis was used to study the dynamic changes of three specific glycoproteins extracted by Triton X-100. The results show that the secretion and synthesis of rabbit endometrial glycoproteins increased remarkably on day 6 (P<0.05). It was found that the WGA-, RCA-I and SBA-binding proteins increased obviously on day 6 and decreased on day 9. The dynamic changes of total and three specific glycoproteins iu endometrium at peri-im-plantation stage indicate that the stage specific glycoproteins are important functionally in key steps of blastocyst implantation.