| 竹叶青蛇毒血小板聚集剂的分离纯化及生化特性 |
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| 引用本文： | 王佐仁,陈军松,吴卫甲,钱嵘,冯波,周元聪.竹叶青蛇毒血小板聚集剂的分离纯化及生化特性[J].中国生物化学与分子生物学报,1996,12(3):303-307. |
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| 作者姓名： | 王佐仁 陈军松 吴卫甲 钱嵘 冯波 周元聪 |
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| 作者单位： | 中国科学院上海生物化学研究所 |
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| 摘 要： | 经ＳｅｐｈａｄｅｘＧ－７５凝胶过滤和ＦＰＬＣＭｏｎｏＱ阴离子交换柱层析及Ｓｕｐｅｒｏｓｅ－１２凝胶过滤，从竹叶青蛇的蛇毒中纯化到一个能诱导人血小板聚集的均一组分．经ＳＤＳ－聚丙烯酰胺凝胶电泳测定其分子量为６８０００左右．等电点为４．３．测定了它的氨基酸组成，对它活化血小板的作用机理进行了初步研究，结果表明它是一种强的血小板激动剂．
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| 关 键 词： | 竹叶青蛇 血小板聚集剂 |
| 收稿时间： | 1996-06-20 |
Purification and Biochemical Characterization of the Platelet Aggregation Factor from the Trimeresurus Stejnegeri Schmidt Venom |
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| Wang Zuo-Ren,ChenJun-Song,Wu Wei-Jia,Qian Rong,Feng Bo,Zhou Yuan-Cong.Purification and Biochemical Characterization of the Platelet Aggregation Factor from the Trimeresurus Stejnegeri Schmidt Venom[J].Chinese Journal of Biochemistry and Molecular Biology,1996,12(3):303-307. |
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| Authors: | Wang Zuo-Ren ChenJun-Song Wu Wei-Jia Qian Rong Feng Bo Zhou Yuan-Cong |
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| Institution: | (Shanghai Institute of Biochemistry, Acdemia Sinica,Shanghai 200031 |
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| Abstract: | A fraction inducing platelet aggregation was isolated and purified from the Trimeresurus stejnegeri Schmidt venom through Sephadex G-75 gel filtration, ion-exchanging chromatography on Mono Q column and gel filtration on Superose-12 column with FPLC. The fraction was proved to be homogenous in SDS-PAGE and IEF. The molecular weight of the fraction estimated by SDS-PAGE was around 68 000,and its isoelectric point detected by IEF was PH 4.3. Amino acid composition of the fraction was determined. The results of its mechanism studied show that it is a potent platelet agonist. |
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| Keywords: | Trimeresurus stejnegeri Schmidt Platelet aggregator |
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