| 抗菌肽Diptericin cDNA的克隆及在E.coli中的融合表达 |
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| 引用本文： | 陈海旭,胡泰山,王新,屈贤铭.抗菌肽Diptericin cDNA的克隆及在E.coli中的融合表达[J].中国生物化学与分子生物学报,2001,17(2):182-184. |
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| 作者姓名： | 陈海旭 胡泰山 王新 屈贤铭 |
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| 作者单位： | 中国科学院上海生物工程研究中心 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 从果蝇成虫中抽提总 RNA,RT- PCR扩增编码伏蝇素 diptericin的 c DNA,克隆并测定了全序列 .将该 c DNA亚克隆到融合表达载体 p MAL- CR1上 ,转化大肠杆菌 BL2 1菌株 ,进行了高效的可溶性融合表达 .通过离子交换层析纯化融合蛋白 ,经 Xa因子酶切后得到的重组 diptericin具有抗菌活性 .
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| 关 键 词： | 伏蝇素 MBP融合蛋白 RT-PCR 融合表达 |
| 收稿时间： | 2001-04-20 |
Cloning and Fusion Expression of Antibacterial Protein Diptericin Gene in Escherichia coli |
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| CHEN Hai xu,HU Tai shan,WANG Xin,QU Xian ming.Cloning and Fusion Expression of Antibacterial Protein Diptericin Gene in Escherichia coli[J].Chinese Journal of Biochemistry and Molecular Biology,2001,17(2):182-184. |
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| Authors: | CHEN Hai xu HU Tai shan WANG Xin QU Xian ming |
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| Institution: | (Shanghai Research Center of Biotechnology,Chinese Academy of Sciences,Shanghai 200233, China |
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| Abstract: | A cDNA encoding diptericin was extracted from Drosophila adults through RT RCR.The cDNA was cloned into pGEM T vector.After restriction analysis and DNA sequencing,the gene was ligated with fusion expression vector pMAL CR1 and transfected into E.coli BL21.The positive clone was induced by IPTG.The expression product was purified by ion exchange chromatography and digested by factor Xa.The final product,rDiptericin,exhibited antibacterial activity. |
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| Keywords: | diptericin MBP fusion protein RT PCR fusion expression |
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