雌激素快速激活MAPK途径促进前列腺间质细胞的增殖

为研究雌激素促进前列腺间质细胞增殖的机理，使用雌二醇（E2）或BSA雌二醇（BSA-E2）处理前列腺间质细胞系wpmy-1，用MTT法及细胞计数法检测细胞增殖情况；用实时RT-PCR以及Western印迹方法检测细胞增殖核抗原（PCNA）的表达水平；用抗磷酸化ERK1/2抗体检测细胞内MAPK途径的激活情况.结果显示，2种形式的雌二醇均能促进前列腺间质细胞系wpmy-1的增殖，并且显著上调增殖相关核抗原PCNA的表达水平；2种形式的雌激素均能快速激活wpmy-1细胞内的MAPK信号通路；MAPK途径的特异性抑制剂PD98059能够显著降低雌激素对细胞增殖以及PCNA表达水平的上调作用.结果表明，雌激素能够通过膜受体快速激活前列腺间质细胞中的MAPK信号通路，进而促进前列腺间质细胞的增殖.

Estrogen Up-regulates the Proliferation of Prostatic Stroma Cells Through Rapid Activation of MAPK Pathway

To investigate the mechanism of estrogen treatment on promoting the proliferation in prostatic stromal cells,a prostatic stromal cell line,wpmy-1,was stimulated with different concentrations of estradiol(E_2)or BSA-estradiol(BSA-E_2).Cell proliferation was monitored by MTT assay and cell counting,and the level of proliferating cell nuclear antigen(PCNA)was measured by real-time RT-PCR and Western blotting.The phosporylation of ERK1/2,an indicator of the activation of MAPK pathway was also determined by Western blotting.We found both E_2 and BSA-E_2 up-regulated the proliferation of wpmy-1 cells and the expression level of PCNA.We also observed a rapid activation of the MAPK pathway following E_2 and BSA-E_2 treatments in wpmy-1 cells.The increase of cell proliferation and PCNA expression induced by estrogen was significantly blocked by PD98059,a selective inhibitor of MAPK pathway.We postulate that estrogen could activate MAPK pathway through its membrane receptor and up-regulate the cell proliferation in prostatic stroma cells.