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| Smad4分子在ERK/MAPK信号转导通路中的作用 | |
| 引用本文： | 何欣蓉,霍艳英,胡迎春,李刚,刘敏丽,宋博强,米粲,吴德昌.Smad4分子在ERK/MAPK信号转导通路中的作用[J].中国生物化学与分子生物学报,2006,22(4):333-337. |
| 作者姓名： | 何欣蓉 霍艳英 胡迎春 李刚 刘敏丽 宋博强 米粲 吴德昌 |
| 作者单位： | 1. 军事医学科学院,附属医院,北京,100850;重庆医科大学,病理学教研室,重庆,400016 2. 军事医学科学院,附属医院,北京,100850 3. 重庆医科大学,病理学教研室,重庆,400016 4. 军事医学科学院,放射医学研究所,北京,100850 |
| 摘 要： | 为了探讨在人永生化支气管上皮细胞BEP2D细胞中，Smad4分子对 ERK/MAPK通路的作用，我们用RNA干扰的方法分别设计了两对Smad4 siRNA，并使BEP2D细胞中Smad4靶向沉默，用Western印迹分析了细胞内ERK激酶和MEK激酶磷酸化水平的变化.结果发现，当Smad4表达沉默后，ERK激酶磷酸化水平未变，MEK激酶磷酸化水平有所降低；再加TGF-β1诱导后ERK激酶和MEK激酶磷酸化水平均显著降低至基础水平以下.结果表明在BEP2D细胞中，Smad4的缺失抑制TGF-β1对ERK/MAPK通路的活化，故提出TGF β活化ERK/MAPK通路需要Smad4存在的假设. |
| 关 键 词： | TGF-β1 Smad4 ERK MEK RNA干涉 |
| 收稿时间： | 2005-8-8 |
| 修稿时间： | 2005年8月8日 |
Smad4 Participates in the TGF-β1-mediated ERK/MAPK Signaling Pathway |
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| HE Xin-Rong,HUO Yan-Ying,HU Ying-Chun,LI Gang,LIU Min-Li,SONG Bo-Qiang,MI Can,WU De-Chang.Smad4 Participates in the TGF-β1-mediated ERK/MAPK Signaling Pathway[J].Chinese Journal of Biochemistry and Molecular Biology,2006,22(4):333-337. | |
| Authors: | HE Xin-Rong HUO Yan-Ying HU Ying-Chun LI Gang LIU Min-Li SONG Bo-Qiang MI Can WU De-Chang |
| Institution: | 1)Insititute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850;2) Department of Pathology,Chongqing University of Medical Science, Chongqing 400016，China;3）The Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100850, China |
| Abstract: | To investigate the function of Smad4 in ERK/MAPK signaling pathway in human bronchial epithelial BEP2D cells. siRNAs were designed to specifically target Smad4 in BEP2D cells. siRNA transfection and western blots were performed to examine the phosphorylation levels of ERK and MEK. The results showed that Smad4 gene silencing leads to the phosphorylation level of MEK1 but not ERK decreased. After stimulation with TGF-β1, phosphorylation levels of both ERK and MEK are decreased and lower than the control cells. The results demonstrated that Smad4 is participating in the activation of the ERK/MAPK signaling by TGF-β1. |
| Keywords: | Smad4 ERK MEK |
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