昆虫杆状病毒晚期启动子在大肠杆菌中启动CAT基因表达

将油桐尺蠖（Ｂｕｚｕｒａｓｕｐｐｒｅｓｓａｒｉａ）核多角体病毒晚期基因──多角体蛋白基因启动子及５′端编码区，以两种不同方式置于缺乏启动子的氯霉素乙酰基转移酶（ＣＡＴ）基因上游，使其分别终止在不同翻译终止位点，其宿主菌具有明显不同的氯霉素抗性，最高达２００ｍｇ／Ｌ　ＬＢ培养基以上，表明昆虫病毒启动子能启动原核基因表达。对多角体蛋白基因启动子能在大肠杆菌中有效工作的原因进行了讨论。

The Expression of CAT Gene in E.coli under the Late Promotor of Insect Baculovirus

The very late gene (polyhedrin gene) promotor of the tung-oil geometrid Buzura suppressaria nuclear polyhedrosis virus (BsNPV) was cloned into the upstream of the promotor-deficit CAT gene of promotor-probe plasmid PKK232-8 with two methods. One of the recombinants pKB9312 had a high resistence to more than 200 mg/L chloramphenicol. This result indicated that insect baculovirus promotor of very late gene may work well in E. coli like that of some animal viruses (e. g., vaccinia virus, adenovirus, etc.). The reason for this phenomenen was discussed.