离心机训练后+Gz耐力相关基因的分离与鉴定

为探讨离心机训练提高正向加速度 (forwardacceleration ,+Gz)耐力的分子机制 ,应用抑制性消减杂交 (suppressionsubtractivehybridization ,SSH)和斑点杂交技术筛选离心机训练 12d后测试高耐力组 (耐受 + 16Gz)与未训练对照组大鼠全脑的差异表达基因 .将获得的序列表达标签 (expressedsequencetag ,EST)进行特异性鉴定后 ,获得 7个上调EST ,其中 5个为已知基因的部分序列 ,2个为新EST ,它们的表达量均在训练 6d组最高 ,表明离心机训练可明显影响大鼠全脑特定基因的表达水平 ,这些基因表达水平的变化很可能与离心机训练提高机体 +Gz耐力的分子机制有关

Isolation and Identification of the Genes Related to +Gz Endurance Improved by Centrifuge Training

To study the molecular mechanism of +Gz endurance improved by centrifuge training. Differentially expressed genes of rat brain between high +Gz endurance (+16 Gz) rats of test group after training for 12 days and control were screened using suppression subtractive hybridization (SSH) and dot blot hybridization. The obtained expressed sequence tags (ESTs) were identified specially. Seven up-regulated ESTs were obtained, among them two are new, and their highest expression levels were occurred in 6 day-trained group. These results showed that centrifuge training can significantly affect the special gene expressions of rat brain, and the expression changes of these genes are possibly related to the mechanism that +Gz endurance can be improved by centrifuge training.