| 鹰嘴豆种子胰蛋白酶抑制剂的分离纯化与鉴定 |
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| 引用本文： | 赵欣,付煊赫,张宗申,刘同祥,王继峰.鹰嘴豆种子胰蛋白酶抑制剂的分离纯化与鉴定[J].中国生物化学与分子生物学报,2011,27(3):287-292. |
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| 作者姓名： | 赵欣 付煊赫 张宗申 刘同祥 王继峰 |
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| 作者单位： | 大连工业大学生物工程学院;北京中医药大学细胞生物化学实验室; |
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| 基金项目： | 国家自然科学基金项目(No.30371775)~~ |
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| 摘 要： | 为了寻找具有药物作用的天然胰蛋白酶抑制物，采用硫酸铵分级沉淀、离子交换层析(DEAE-纤维素52)及Sephadex G-100凝胶层析等方法, 从鹰嘴豆种子中分离出一种鹰嘴豆胰蛋白酶抑制剂（CPTI）. 研究表明：CPTI对胰蛋白酶有较强的抑制作用,抑制率达80%,而对胰凝乳蛋白酶抑制作用较弱,抑制率为32%, 对胃蛋白酶、木瓜蛋白酶及枯草杆菌蛋白酶均无抑制作用; 用SDS-PAGE测得CPTI近似分子质量为25.7 kD; CPTI具有较高的热稳定性,在100 ℃下加热60 min,对胰蛋白酶活性仍保持78%抑制率; Lineveaer-Burk作图得知该抑制剂属竞争性抑制类型. 动力学测定显示，来自鹰嘴豆中的CPTI对胰蛋白酶的抑制作用常数(Ki)为3.99×10-7 mol/L.
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| 关 键 词： | 鹰嘴豆 胰蛋白酶抑制剂 分离纯化 鉴定 |
| 收稿时间： | 2010-09-09 |
Purification and Characterization of Trypsin Inhibitor from Chickpea Seeds |
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| ZHAO Xin,FU Xuan-He,ZHANG Zong-Shen,LIU Tong-Xiang,WANG Ji-Feng School of Biological Engineering,Dalian Polytechnic University,Dalian ,China, Laboratory of Cell , Biochemistry,Beijing University of Chinese Medicine,Beijing ,China.Purification and Characterization of Trypsin Inhibitor from Chickpea Seeds[J].Chinese Journal of Biochemistry and Molecular Biology,2011,27(3):287-292. |
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| Authors: | ZHAO Xin FU Xuan-He ZHANG Zong-Shen LIU Tong-Xiang WANG Ji-Feng School of Biological Engineering Dalian Polytechnic University Dalian China Laboratory of Cell Biochemistry Beijing University of Chinese Medicine Beijing China |
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| Institution: | ZHAO Xin1),FU Xuan-He1),ZHANG Zong-Shen1),LIU Tong-Xiang2),WANG Ji-Feng2)(1) School of Biological Engineering,Dalian Polytechnic University,Dalian 116034,China,2) Laboratory of Cell and Biochemistry,Beijing University of Chinese Medicine,Beijing 100029,China) |
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| Abstract: | Chickpea trypsin inhibitor(CPTI) was purified and characterized from the seeds of Cicer arietinum L using ammonium sulfate grade precipitation,anion exchange chromatography(DEAE-cellulose 52),and gel filtration on Sephadex G-100.The extraction from Cicer arietinum L seeds strongly inhibit trypsin activity by 80%,and inhibit chymotrypsin activity by 32%,but exhibited no effects on three proteases of pepsin,papain and subtilisin.The approximate molecular weight of CPTI was estimated to be 25.7 kD by SDS-PAGE.... |
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| Keywords: | Cicer arietinum L chickpea trypsin inhibitor isolation and purification characterization |
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