| 根癌农杆菌介导转化谷子的影响因素 |
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| 引用本文： | 刘颖慧,于静娟,敖光明,赵倩.根癌农杆菌介导转化谷子的影响因素[J].中国生物化学与分子生物学报,2007,23(7):531-536. |
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| 作者姓名： | 刘颖慧 于静娟 敖光明 赵倩 |
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| 作者单位： | 1. 中国农业大学生物学院,北京,100094;河北北方学院,张家口,075000
2. 中国农业大学生物学院,北京,100094 |
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| 基金项目： | 国家转基因植物研究和产业化专项基金 |
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| 摘 要： | 利用GUS报告基因,建立了农杆菌介导的谷子(Setaria italica)遗传转化体系,并研究了多种影响因素对转化效率的影响,包括受体基因型、外植体类型、菌液浓度、培养基中乙酰丁香酮的浓度、侵染时间和共培养时间.确定了最优的转化条件为:以谷子幼穗诱导的愈伤组织为外植体,用低浓度的农杆菌菌液侵染30～40min,然后在含有0.1mmol/L乙酰丁香酮的LS培养基上共培养2d.
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| 关 键 词： | 谷子 转化效率 农杆菌介导转化 影响因素 |
| 修稿时间： | 2006年11月30 |
Factors Influencing Agrobacterium-mediated Transformation of Foxtail Millet (Setaria italica) |
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| LIU Ying-Hui,YU Jing-Juan,AO Guang-Ming,ZHAO Qian.Factors Influencing Agrobacterium-mediated Transformation of Foxtail Millet (Setaria italica)[J].Chinese Journal of Biochemistry and Molecular Biology,2007,23(7):531-536. |
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| Authors: | LIU Ying-Hui YU Jing-Juan AO Guang-Ming ZHAO Qian |
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| Abstract: | An efficient and repeatable approach in transforming the foxtail millet (Setaria italica ) using Agrobacterium LBA4404 horboring the plasmid pBI121 was established. Factors affecting transformation efficiency were investigated including the genotype, explants, the density of bacteria, the duration for inoculation and co-cultivation, and the concentration of acetosyringone in the medium. The maximum transformation conditions were: the callus induced from inflorescence was used as explant; the duration for inoculation with Agrobacterium at low cell density was 30-40 min; for the co-cultivation , the suitable concentration of acetosyringone in the medium was 0.1 mmol/L, and the duration was 2 days. |
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| Keywords: | foxtail millet transformation frequency Agrobacterium-mediated transformation influence factors |
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