| p53基因对人胃癌细胞系恶性生长的影响 |
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| 引用本文： | 王培新,王新娟,高汉林.p53基因对人胃癌细胞系恶性生长的影响[J].中国生物化学与分子生物学报,1997,13(1):46-49. |
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| 作者姓名： | 王培新 王新娟 高汉林 |
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| 作者单位： | 北京医科大学生物化学与分子生物学系!北京，100083，北京医科大学生物化学与分子生物学系!北京，100083，北京医科大学生物化学与分子生物学系!北京，100083 |
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| 摘 要： | 以p53cDNA为探针，用Southern印迹法对人胃癌细胞系BGC823进行了检测，发现该细胞中P53基因存在异常．将可在真核细胞表达的重组野生型P53质粒PC53一SN3和突变型P53质粒PC53—SCX3，用脂质体介导法，分别导入BGC823细胞，获得了较长时间耐受G418的多个阳性克隆．Southern印迹法证实阳性克隆细胞中有外源性P53基因存在．比较BGC823细胞，转染野生型及突变型P53质粒的3种细胞生长曲线和软琼脂集落形成状况发现，野生型P53基因对BGC823细胞恶性生长有一定抑制作用．
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| 关 键 词： | P53 人胃癌细胞系 基因转染 细胞生长曲线 软琼脂集落形成 |
| 收稿时间： | 1997-02-20 |
The effect of p53 on the Malignant Growth of Human Gastric Carcinoma Cell Line BGC823 |
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| Wang Pei-Xin,Wang Xin-Juan,Gao Han-Lin,Liao Xiao-Bei,Lian Xin-Yun.The effect of p53 on the Malignant Growth of Human Gastric Carcinoma Cell Line BGC823[J].Chinese Journal of Biochemistry and Molecular Biology,1997,13(1):46-49. |
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| Authors: | Wang Pei-Xin Wang Xin-Juan Gao Han-Lin Liao Xiao-Bei Lian Xin-Yun |
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| Institution: | (Department of Biochemistry and Molecular Biology, Beijing MedicalUniversity, Beijing 100083 |
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| Abstract: | Compared with normal gastric mucous tissue, aberrant fragments of p53 gene werefound in human gastric carcinoma cell line BGC823 by Southern blotting analysis. Recombinantplasmids, PC53-SN3 and PC53-SCX3, were transfected into BGC823 cells mediated by lipofectin. G418-resistant colonies were obtained after growing a period of time under selected pressureof G418. Southern blotting analysis confirmed that exogenous p53 gene existed in the host cell.Cell growth curve and soft agar colony formation experiment indicated that wild type p53 genehad some extent negative effect on malignant growth of the cell line BGCB23. |
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| Keywords: | p53 Human gastric carcinoma cell line Cell growth curve Soft agar colony formation Gene transfection |
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