| 一种用于蛋白质C端序列测定的脯氨酸乙内酰硫脲(TH-Pro)新制备方法 |
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| 引用本文： | 莫碧兰,李江,梁宋平.一种用于蛋白质C端序列测定的脯氨酸乙内酰硫脲(TH-Pro)新制备方法[J].中国生物化学与分子生物学报,1998,14(2):186-191. |
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| 作者姓名： | 莫碧兰 李江 梁宋平 |
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| 作者单位： | 湖南师范大学生物系 |
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| 摘 要： | 采用Ｌ－型脯氨酸（Ｌ－Ｐｒｏ）作为原料，三甲基硅烷异硫氰酸酯（ＴＭＳ－ＩＴＣ）作为偶联试剂制备脯氨酸乙内酰硫脲（ＴＨ－Ｐｒｏ）．产物经反相ＨＰＬＣ分离纯化，并通过氨基酸组成分析，紫外光谱扫描，质谱和核磁共振等方法鉴定．反应产率高达９６％．
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| 关 键 词： | 脯氨酸乙内酰硫脲 蛋白质C端序列分析 氨基酸组成分析 质谱 核磁共振 |
| 收稿时间： | 1998-04-20 |
An Efficient and Convenient Approach to Prepare Proline Thiohydantoin for Development of C terminal Sequencing |
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| Mo Bi,Lan\ Li Jiang\ Liang Song,Ping.An Efficient and Convenient Approach to Prepare Proline Thiohydantoin for Development of C terminal Sequencing[J].Chinese Journal of Biochemistry and Molecular Biology,1998,14(2):186-191. |
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| Authors: | Mo Bi Lan\ Li Jiang\ Liang Song Ping |
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| Institution: | (Biology Department of Hunan Normal University,Changsha 410081 |
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| Abstract: | A new method to prepare proline thiohydantoin which is required as reference standard for development of C terminal sequencing is reported.Proline thiohydantoin was prepared using a straightforward method involving reaction of proline with trimethylsilylisothiocyanate(TMS ITC).The product was characterized by amino acid analysis,UV spectrum,mass spectrometry and NMR.Different reaction conditions were investigated and the chemical mechanism scheme of proline thiohydantoin is presented.The yield of proline thiohydantoin reached up to 96%. |
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| Keywords: | Proline thiohydantoin C terminal sequencing Amino acid analysis Mass spectrometry NMR |
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