| 壳聚糖固定化木瓜蛋白酶的研究 |
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| 引用本文： | 姜涌明,隋德新,赵国骏,史永昶,施伟.壳聚糖固定化木瓜蛋白酶的研究[J].中国生物化学与分子生物学报,1993,9(4):470-474. |
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| 作者姓名： | 姜涌明 隋德新 赵国骏 史永昶 施伟 |
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| 作者单位： | 扬州大学农学院酶工程研究室，扬州大学农学院酶工程研究室，扬州大学农学院酶工程研究室，扬州大学农学院酶工程研究室，扬州大学农学院酶工程研究室 扬州 225001，扬州 225001，扬州 225001，扬州 225001，扬州 225001 |
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| 基金项目： | 农业部生08-03课题 |
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| 摘 要： | 以自制的壳聚糖作为载体,用戊二醛作交联剂,优化了固定化条件,研制成壳聚糖固定化木瓜蛋白酶。其活性回收率达到42—53％,操作半衰期达到一个月以上,对热、乙醇以及尿素的稳定性有很大的提高,Km值为0.67×10~2mg/mL,最适温度65—70℃,最适pH8.0,能使啤酒中的蛋白质浓度从56.5mg/L减少到2.7mg/L,可以消除啤酒的低温混浊现象。
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| 关 键 词： | 木瓜蛋白酶 壳聚糖 固定化 |
| 收稿时间： | 1993-08-20 |
Studies on Immobilization of Papain on Chitosan |
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| Jiang,Yong-ming Shui,De-xin Zhao,Guo-jun Shi,Yong-chang Shi,Wei.Studies on Immobilization of Papain on Chitosan[J].Chinese Journal of Biochemistry and Molecular Biology,1993,9(4):470-474. |
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| Authors: | Jiang Yong-ming Shui De-xin Zhao Guo-jun Shi Yong-chang Shi Wei |
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| Institution: | (Laboratory of Enzyme Engineering, Agricultural College, Yangzhou University Yangzhou, 225001 |
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| Abstract: | We used chitosan as support, glutaraldehyde as croaslinking agent to prepare immobilized papain. The conditions of immobilization were improved. The activity recovery of immobilized papain reached 42—53%. The operation half-life time was 30 days. Stability of immobilized enzyme to temperature, ethanol and urea was increased obviously compared, to that of soluble enzyme. The Km value of the immobilized enzyme was 0.67×10~2mg/mL and the optimum temperature and optimum pH was 65—70℃ and 8.0 respectively. The immobilized papain could make the protein concentration of beer decrease from 56.5mg/L to 2.7mg/L, thus the pheno menon of turning turbid upon chilling was avoided. |
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| Keywords: | Papain Chitosan Immobilization |
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