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| 重组人磷脂酶D2在体内能明显抑制慢性哮喘豚鼠血清中GPI-PLD的表达 | |
| 引用本文： | 朱玲,余传星,邹伟斌,何小丽,林俊锦.重组人磷脂酶D2在体内能明显抑制慢性哮喘豚鼠血清中GPI-PLD的表达[J].中国生物化学与分子生物学报,2007,23(2):116-121. |
| 作者姓名： | 朱玲 余传星 邹伟斌 何小丽 林俊锦 |
| 作者单位： | 1. 福建医科大学基础免疫系及神经生物学研究中心,福州,350004 2. 福建省第二人民医院内科,福州,350003 |
| 基金项目： | 福建省科技攻关项目;福建省产业技术研究开发项目 |
| 摘 要： | 通过建立慢性豚鼠哮喘模型,研究重组人磷脂酶D2(rhPLD2)干预慢性哮喘豚鼠血清中糖基化磷脂酰肌醇特异性磷脂酶D(GPI-PLD)含量的变化，探寻可能的机制. 以rhPLD2干预慢性哮喘豚鼠，用TX-114分相法检测豚鼠血清中GPI-PLD酶活性.与正常状态豚鼠相比较，慢性哮喘状态豚鼠其血清中GPI-PLD酶活性显著升高.但以rhPLD2及地塞米松干预慢性哮喘豚鼠后，该指标显著下降. rhPLD2可通过抑制其血清中GPI-PLD酶活性表达,来抑制哮喘发生过程中的炎症反应. |
| 关 键 词： | 重组人磷脂酶D2 糖基化磷脂酰肌醇特异性磷脂酶D 慢性哮喘 豚鼠 |
| 收稿时间： | 2006-8-16 |
| 修稿时间： | 2006年8月16日 |
Recombinant Human PLD2(rhPLD2)May Significantly Inhibit Expression of GPI-PLD of Guinea Pigs of Chronic Asthma in vivo |
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| ZHU Ling,YU Chuan-Xing,ZOU Wei-Bin,HE Xiao-Li,LIN Jun-Jin.Recombinant Human PLD2(rhPLD2)May Significantly Inhibit Expression of GPI-PLD of Guinea Pigs of Chronic Asthma in vivo[J].Chinese Journal of Biochemistry and Molecular Biology,2007,23(2):116-121. | |
| Authors: | ZHU Ling YU Chuan-Xing ZOU Wei-Bin HE Xiao-Li LIN Jun-Jin |
| Institution: | Deparment of Immunology and Center of Neuroscience, Fujian Medical University, Fuzhou 350004,China; Internal Medicine of Second People’s Hospital of Fujian Province,Fuzhou 350003, China |
| Abstract: | The effect of recombinant human phospholipase D2(rhPLD2)in vivo was investigated on the secretion of serum glycosyl phosphatidylinositol-specific phospholipase D(GPI-PLD)in guinea pigs of chronic asthma．Ater treating the guinea pigs attacked by chronic asthma with rhPLD2,the GPI-PLD activity detection was canrried out by phase separation of human placental alkaline phosphatase in Triton X-114．Compared with the healthy guinea pigs(NS group),the serum GPI-PLD in the guinea pigs of chronic asthma are much higher than that of control groups,P≤0．01．Our results showed that rhPLD2 could significantly reduce the secretion of GPI-PLD when the guinea pigs were attacked by chronic asthma． |
| Keywords: | rhPLD2 GPI-PLD chronic asthma guinea pig |
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