| 类泛素化修饰蛋白SUMO1的表达纯化及抗体制备 |
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| 引用本文： | 祖玲玲,史颖娇,秦,宇,王远根,王照娜,王颖.类泛素化修饰蛋白SUMO1的表达纯化及抗体制备[J].中国生物化学与分子生物学报,2008,24(11):1029-1033. |
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| 作者姓名： | 祖玲玲 史颖娇 秦 宇 王远根 王照娜 王颖 |
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| 作者单位： | 南开大学泰达生物技术学院 |
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| 摘 要： | SUMO是近年发现的类泛素化修饰蛋白，可通过异肽键共价连接到靶蛋白上，影响靶蛋白的细胞内定位、稳定性及与其它生物大分子的相互作用. 为研究蛋白质的SUMO化修饰，本文表达并利用亲和层析的方法纯化了重组的人SUMO1，制备了兔抗hSUMO1的多克隆抗体. 经ELISA和免疫印迹检测，获得了灵敏度高、特异性好的抗体，可用于SUMO化修饰靶蛋白的鉴定及SUMO化修饰的生物学功能研究.
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| 关 键 词： | SUMO1 表达纯化 多克隆抗体 |
| 收稿时间： | 2008-4-18 |
| 修稿时间： | 2008-6-27 |
Overexpression and purification of human SUMO1 and the generation of antibody against human SUMO1 |
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| WANG Ying.Overexpression and purification of human SUMO1 and the generation of antibody against human SUMO1[J].Chinese Journal of Biochemistry and Molecular Biology,2008,24(11):1029-1033. |
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| Authors: | WANG Ying |
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| Institution: | 1)TEDA School of Biological Sciences and Biotechnology, Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, and Tianjin Key Laboratory of Microbial Functional Genomics, Nankai University, Tianjin 300457,China;2) College of Basic Medical Science, Tianjin Medical University, Tianjin 300070, China |
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| Abstract: | Small ubiquitin-like modifier (SUMO) is a recent member of ubiquitin-like protein superfamily. As a form of post-translational modification, SUMO attached covalently to target proteins to alter the localization, stability of the target proteins, as well as its interactions with other proteins. To investigate protein SUMOylation, we expressed and purified human SUMO1, and used to raise high-titer SUMO1-specific polyclonal antiserum in rabbits. The obtained antibodies can recognize endogenous SUMO1 and its association with target protein, thus can be used for the identifications of SUMO target proteins and the studies on biological functions of SUMOylation. |
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| Keywords: | SUMO1 expression and purification polyclonal antibody |
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