| 以融合蛋白形式在大肠杆菌中表达人MCP－1 |
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| 引用本文： | 叶棋浓,苏国富,徐永强,黄翠芬.以融合蛋白形式在大肠杆菌中表达人MCP－1[J].中国生物化学与分子生物学报,1995,11(2):146-149. |
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| 作者姓名： | 叶棋浓 苏国富 徐永强 黄翠芬 |
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| 作者单位： | 军事医学科学院生物工程研究所 |
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| 摘 要： | 将编码人单核细胞趋化蛋白－１（ＭＣＰ－１）的基因亚克隆到大肠杆菌表达载体ｐＥＸ３１Ａ中，在大肠杆菌中表达出ＭＳ２／ＭＣＰ－１融合蛋０白，该表达产物约占菌体总蛋白的１５％左右，Ｗｅｓｔｅｒｎｂｌｏｔ检测表明，表达产物可与ＭＣＰ－１抗体特异反应。采用琼脂糖平板法进行活性测定表明，表达产物具有明显的单核细胞趋化活性，说明Ｎ端融合一段细菌蛋白对ＭＣＰ－１有无趋化活性可能没有影响。
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| 关 键 词： | 人单核细胞趋化蛋白－1 基因的克隆与表达 融合蛋白 |
| 收稿时间： | 1995-04-20 |
Expression of MCP-1 in the Form of Fusion Protein in E.coli |
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| Ye Qi-Nong,Su Guo-Fu,Xu Yong-Qiang,Huang Cui-Feng.Expression of MCP-1 in the Form of Fusion Protein in E.coli[J].Chinese Journal of Biochemistry and Molecular Biology,1995,11(2):146-149. |
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| Authors: | Ye Qi-Nong Su Guo-Fu Xu Yong-Qiang Huang Cui-Feng |
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| Institution: | (Institute of Biotechnology, Beijing 100850 |
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| Abstract: | A gene fragment encoding human monocyte chemoattratctant protein-1 (MCP-1) was subcloned into pEX31A. MS2/MCP-1 fusion protein was expressed in E. coli and accounted for about 15% of total cell proteins. Western blot analysis indicated that the expression product reacted specifically with anti-MCP-1 antibodies. Detection of activity by the method of agarose plates showed that the expression product had obvious monocyte chemoattractant activity, suggesting that bacterial protein at N-terminus may not affect the chemoattractant activity of MCP-1. |
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| Keywords: | Human monocyte chemoattractant protein-1 Gene cloning and expression Fusion protein |
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