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小鼠Lewis肺癌组织中层粘连蛋白受体的分离及其性质的研究
引用本文:王苏,周柔丽.小鼠Lewis肺癌组织中层粘连蛋白受体的分离及其性质的研究[J].中国生物化学与分子生物学报,1988,4(3):218-226.
作者姓名:王苏  周柔丽
作者单位:北京医科大学细胞研究室 (王苏),北京医科大学细胞研究室(周柔丽)
摘    要:层粘连蛋白(Laminin,LN)是基膜(basement membrane)中的一种主要大分子糖蛋白。一些研究资料表明肿瘤细胞的浸润转移可能与LN有关。肿瘤细胞与LN的作用可能是通过细胞表面LN受体进行的。本文采用亲和层析法从小鼠Lewis肺癌组织中分离LN受体并对其理化性质进行研究。Lewis肺癌LN受体的表观分子量为70,000,还原后SDS电泳图为一条较宽的条带。氨基酸组成中疏水氨基酸占38%,苏氨酸、絲氨酸、门冬氨酸(包括门冬酰胺)占23.5%,通过硝酸纤维素膜片法用HRP-LN测定受体与LN的结合特性,证明具有配基结合专一性,饱和性及高亲和性(Kd=0.95×10~(-9)mol/L)。

关 键 词:层粘连蛋白  层粘连蛋白受体  HRP-LN  Lewis肺癌  Ⅳ型胶原  
收稿时间:1988-06-20

PURIFICATION AND CHARACTERIZATION OF A LAMININ RECEPTOR FROM MURINE LEWIS LUNG CARCINOMA TISSUE
Wang,Su Zhou,Rou-li.PURIFICATION AND CHARACTERIZATION OF A LAMININ RECEPTOR FROM MURINE LEWIS LUNG CARCINOMA TISSUE[J].Chinese Journal of Biochemistry and Molecular Biology,1988,4(3):218-226.
Authors:Wang  Su Zhou  Rou-li
Institution:(Department of Cell Biology, Beijing Medical University, Beijing
Abstract:Laminin (LN) is a macromolecular glycoprotein in basement membranes. previous Studies suggest that LN may be involved in the invasion and metastasis of cancer cells. The biological functions of LN may be performed by LN receptors on cell surface. We isolated the LN receptor from the tissue of Lewis lung carcinoma by affinity chromatography. It appeared as a single band on SDS-PAGE after reduction. The molecular weight was about 70,000. The amino acid analysis showed that the LN receptor was composed of 38% of hydrophobia amino acids and among the hydrophilic amino acids 23.5% were made of Thr, Ser and Asp (including Asn).Nitrocellulose disk assay demonstrated that the binding of HRP-LN to the LN receptor was specific, saturable, and high in affinity(Kd = 0.95 ×10-9mol/L).
Keywords:Laminin(LN) LN receptors HRP-LN Lewis lung carcinoma Type IV Collagen    
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