麦芽酚对活性氧损伤人神经瘤细胞的保护作用

以人神经瘤细胞株 (SH SY5Y)为材料 ,使用过氧化氢 (H2 O2 )产生过量活性氧诱导SH SY5Y细胞株进入氧化应激状态 .研究麦芽酚对过量活性氧造成的SH SY5Y细胞株氧化损伤的保护作用 .分析活性氧对细胞膜蛋白和DNA的损伤 ,细胞线粒体功能变化 ,白介素 6 (IL 6 )的表达变化以及细胞核因子κB(NF κB)的激活 .结果显示 ,2mmol L麦芽酚保护细胞 2h后 ,对细胞膜蛋白和DNA的损伤均有明显的保护作用 ,减少了膜蛋白的氧化和细胞DNA片段化的形成 ,细胞线粒体功能损伤减小 ,细胞表达的IL 6减少 ,被激活的NF κB水平同时降低 .结果证明 ,麦芽酚可以有效保护活性氧对神经细胞的氧化损伤 ,维持细胞的正常生理功能

Protection of Human Neuroblastoma Cells Against Oxidative Damage by Maltol

The protection of maltol on human neuroblastoma cell line (SH SY5Y)was investigated when cells were treated with reactive oxygen species. Human neuroblastoma cell line SH SY5Y was pretreated with 2 mmol/L maltol for 2 hours and then treated with hydrogen peroxide. The damages of membrane proteins and DNA, mitochondrial function, activation of NF κB and expression of IL 6 were studied. The damages of membrane proteins, DNA of cells and mitochondrial function were reduced, expression of IL 6 was also reduced and activation of NF κB was lower as compared with that without pretreatment by maltol. Maltol was shown to protect SH SY5Y cell from oxidative damage and maintain its normal functions.