| 大肠杆菌表达重组人白细胞介素-9的纯化及部分性质 |
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| 引用本文： | 张津辉,蒋中华,陈惠鹏,魏汉东,邢桂春,贺福初.大肠杆菌表达重组人白细胞介素-9的纯化及部分性质[J].中国生物化学与分子生物学报,1998,14(5):562-566. |
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| 作者姓名： | 张津辉 蒋中华 陈惠鹏 魏汉东 邢桂春 贺福初 |
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| 作者单位： | 军事医学科学院放射医学研究所 |
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| 摘 要： | 表达重组人白细胞介素－９（ｒｅｃｏｍｂｉｎａｎｔｈｕｍａｎｉｎｔｅｒｌｅｕｋｉｎ－９ｒｈＩＬ－９）的大肠杆菌经破碎、包涵体洗涤、裂解提取、凝胶过滤和离子交换色谱分离，得到了电泳纯的ｒｈＩＬ－９，回收率６６％，分子量与理论值相符，有刺激小鼠骨髓巨核系集落生成的活性．为ｒｈＩＬ－９的大规模制备及更深入的研究奠定了基础
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| 关 键 词： | 白细胞介素－9 色谱法 蛋白质纯化 |
| 收稿时间： | 1998-10-20 |
Purification and Some Characteristics of Recombinant Human Interleukin 9 Expressed in E.coli |
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| Zhang Jin,Hui,Jiang Zhong,Hua,Chen Hui,Peng,Wei Han,Dong,Xing Gui,Chun,He Fu,Chu.Purification and Some Characteristics of Recombinant Human Interleukin 9 Expressed in E.coli[J].Chinese Journal of Biochemistry and Molecular Biology,1998,14(5):562-566. |
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| Authors: | Zhang Jin Hui Jiang Zhong Hua Chen Hui Peng Wei Han Dong Xing Gui Chun He Fu Chu |
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| Institution: | (Beijing Institute of Radiation Medicine,Beijing 100850 |
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| Abstract: | Recombinant human interleukin 9(rhIL 9) expressed in E.coli was isolated and purified by washing and solubilization of inclusion body,gel filtration chromatography and ion exchange chromatography.The final product displayed a single band with a corresponding purity of 99.2% in SDS PAGE stained by Coomassie brilliant blue R 250,and the total recovery of rhIL 9 was 66%.Molecular weight of rhIL 9 determined by SDS PAGE was 15.5kD.Biological activity analysis showed that rhIL 9 increased CFU Meg formation of normal mouse bone marrow cells.This study provided the basis for large scale preparation and further research of rhIL 9. |
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| Keywords: | Interleukin 9 Chromatography Protein purification |
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