Cre/lox位点特异重组系统在转基因植物中的应用

位点特异重组系统由重组酶和相应的重组酶识别位点组成,通过两者间的相互作用,实现外源基因精确整合与切除等一系列遗传操作.主要可分为Cre/lox系统、FLP/frt系统、R/RS系统和Gin/gix系统.目前,研究最充分应用最广泛的位点特异重组系统为Cre/lox系统.此系统为位点特异重组系统家族中的一员,由38.5kDCre重组酶和34bplox位点组成,最早被应用于动物转基因研究,包括基因敲除、基因激活、基因易位等.近年来,随着研究的深入,Cre/lox系统被逐步应用到植物研究中,并在诸多领域取得重大进展.本文总结归纳了Cre/lox系统在定点整合、定点切除以及叶绿体转化等方面的最新研究成果,旨在为利用Cre/lox系统构建环境安全和高效表达的植物遗传转化体系提供参考.

Application of Cre/lox Site-specific Recombination Systemin Transgenic Plants

Site-specific recombination system, which consists of recombinase and the related-recombinase recognizing short DNA sequences, has been developed into genetic manipulation tool by two-component interaction for the goal of targeted integration and precise excision of DNA fragment. Cre/lox recombination system, which belongs to the family of site-specific recombination system including Cre/lox, FLP/frt, R/RS and Gin/gix, is one of the best characterized and most widely used system. Cre/lox system consists of Cre recombinase and 34 bp lox sites, which initially was applied in basic transgenic animal research such as gene knockout, gene activation and gene translocation. With the rapid development of biotechnology, Cre/lox system has been investigated to transgenic plants and makes impressive progress in recent years. In this paper, the advances of Cre-lox system are reviewed, providing insight of site-specific integration, site-specific excision and plant chloroplast transformation to construct environmental friendly plant genetic transformation system with high-level foreign gene expression.