视黄酸挽救的视黄酸合成酶Raldh2基因敲除鼠E10.75胚胎后肢发育正常

视黄酸合成酶Raldh2基因敲除鼠胚胎没有肢体的发育在胚胎E6.75-E 8.25期间，喂给怀孕母鼠含视黄酸（0.1 mg/g食物）食物后，Raldh2基因敲除鼠E10.75胚胎后肢形态正常，前肢发育较小.原位杂交结果表明,决定肢体近 远端轴发育的标志基因(marker gene)Fgf8，决定前-后轴发育的标志基因Shh以及后肢发育特异性基因Tbx4 和Pitx1在视黄酸挽救的Raldh2基因敲除鼠E10.75胚胎的后肢表达正常.上述结果提示,视黄酸可以挽救Raldh2基因敲除鼠E10.75胚胎后肢的正常发育.

Raldh2 knock-out Embryos at E10.75 Rescued by Retinoic Acid Develop Normal Hindlimbs

Retinaldehyde dehydrogenase-2(RALDH2)catalyzes retinoic acid(RA)synthesis.Raldh2 knock-out embryos have no limb buds development,but their embryos at E10.75 develop small forelimbs and morphological normal hindlimbs when pregnant female Raldh2 heterozygous knock-out mice were rescued by the food containing retinoic acid(0.1 mg/g food)from E6.75 to E8.25.The results of hybridization in situ showed that the expression of proximo-distal marker gene Fgf8,antero-posterior marker gene Shh and hindlimb specific marker gene Tbx4 and Pitx1 was normal in the hindlimbs of RA rescued Raldh2 knock-out embryos at E10.75.This indicated that RA can rescue the normal hindlimbs development of Raldh2 knock-out embryo at E10.75.