丹参酮lI-A磺酸钠及莨菪等药物对红细胞膜稳定性及钙调节蛋白(CaM)功能的影响

 为了进一步证实某些药物对红细胞膜稳定(即抗溶血)作用及抑制CaM功能与药理作用之间的相关性,本文选用丹参酮Ⅱ-A磺酸钠,戊脉安,奎尼丁和莨菪类药物,测定它们对大鼠红细胞溶血及CaM功能的影响。结果表明丹参酮Ⅱ-A磺酸钠,戊脉安,奎尼丁对红细胞溶血抑制率分别为100％,71％,32％,对CaM功能的抑制分别为71.8％,63.4％,46.4％。莨菪类药物有促溶血作用,对CaM功能的抑制也极弱。我们还发现以上药物对膜上Ca~(2+)+Mg~(2+)-ATP酶的直接作用亦有类似的规律。这种相关性可能与膜表面CaM与钙离子,靶酶及药物结合区的疏水性及药物本身的相对疏水性有关。

EFFECT OF TANSHINONE Ⅱ-A SUIFONATE, HYOSCYAMUS ON ERYTHROCYTE MEMBRANE AND CAM FUNCTION

It has been reported that drugs in certain pharmacological classes share two interesting properties. the properties are: 1. Inhibition of the function of a calcium-activated protein called calmodulin, and 2. Protection of erythrocytes against osmotic lysis. The classes of drugs that share those properties include cardiac antiarrhysmic agents.The present study was designed to determine whether there is an association of drug-induced inhibition of calmodulin functions, drug-induced membrane stabilization, and pharmacological effects of Tanshinone Ⅱ-A. sulfonate, Hyos-cyamus. It has been reported that Tanshinone Ⅱ-A sulfonate has a strong effect on protection of erythrocyte against different kinds of hemolysis. We found that Tanshinone Ⅱ-A. sulfonate is a strong inhibitor to CaM function. These data may explain the machanism of the clinical effects of the drug.