短裙竹荪多糖Dd-S3P的分离纯化及其性质研究

短裙竹荪子实体经2％Na2CO3溶液提取，用蛋白酶法和Sevag法相结合除去蛋白，乙醇分级沉淀，级分3经DEAE－SephadexA－25柱层析纯化得到短裙竹蒸蒸荪多糖DdS3P．经测定该多糖为均一组分，分子量约为3．8×105，红外光谱呈现出典型的多糖吸收峰，含有α-型糖苷连接键，紫外扫描无核酸和蛋白质的特征吸收峰．纸层析和气相层析分析得知Dd－S3P含有D-葡萄糖（D－Glc）、D-甘露糖（D－Man）和D-木糖（D－Xyl），其摩尔比为1．83：1．00：1．21．经动物试验表明此多糖对小鼠S-180有一定的抑制作用，抑瘤率约31．3％．

Isolation, Purification and Characterization of Polysaccharide Dd-S3P from Dictyophora duplicata (Bosc) Fischer

A polysaccharide Dd-S3P was extracted from the dried fruiting bodies of Dictyophoraduplicata by 2 % Na2CO3 solution. The extract was deproteinized by pronase treatment and Sevagmethod, then precipitated by fractional precipitation with ethanol. Dd-S3P was further purified byDEAE-Sephadex A-25 chromatography column. Dd-S3P was proved to be homogeneous by gel filtration and polyacrylamide gel electrophoresis. The molecular weight of Dd-S3P was estimated tobe 380 000. No characteristic absorption of nucleic acid and protein was shown in Dd-S3P UVspectrum, but typical absorption of polysaccharide was shown in its IR spectrum. It contained abonds by IR analysis. The components of Dd-S3P were identified by paper and gas chromatographies, which was composed of D-glucose, D-mannose and D-xylose with the molar ratioof 1. 83: 1. 00: 1. 21. The inhibition rate of Dd-S3P against subcutaneously implanted Sarcoma 180in mice was 31. 3 %.