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生理性及病理性人体甲种胎儿蛋白分子异质性研究
引用本文:许凯黎,周瑾,黄抗美.生理性及病理性人体甲种胎儿蛋白分子异质性研究[J].中国生物化学与分子生物学报,1986,2(3):7-12.
作者姓名:许凯黎  周瑾  黄抗美
作者单位:上海市肿瘤研究所 (许凯黎,周瑾),上海医科大学肿瘤医院(黄抗美)
摘    要:为阐明人体组织处于生理及病理状态时合成及分泌的各种甲种胎儿蛋白(AFP)分子中糖基组成上的差异,本文采用刀豆球蛋白A(ConA)及扁豆凝集素(LCA)亲和双向放射免疫电泳(Aff-RIEP)分析人体羊水、孕妇、急性肝炎及原发性肝癌患者血清中各种不同来源的AFP。实验表明孕妇及肝炎血清中AFP在ConA及LCA电泳中呈现相似的电泳图谱,但与肝癌AFP有显著差异。在ConA电泳中,肝癌血清显示二种分子变异体,其中以ConA结合型为主,兼有小部分非结合型AFP,而肝炎及孕妇AFP仅为一种ConA结合型分子。然而,在LCA电泳中,肝炎及孕妇血清AFP却以LCA非结合型为主,兼有小部分结合型,但肝癌AFP却与之相反,以结合型为主,非结合型为次。上述结果提示癌性与非癌性AFP分子之间存在糖基组成及结构上的差异。

关 键 词:甲种胎儿蛋白  AFP分子变异体  LCA亲和双向放射免疫电泳  病理和生理状态  
收稿时间:1986-06-20

STUDY ON MOLECULAR HETEROGENEITY OF ALPHA-FETOPROTEIN IN PHYSIOLOGICAL AND PATHOLOGICAL STATE
Xu,Kai-Li Zhou,Jin.STUDY ON MOLECULAR HETEROGENEITY OF ALPHA-FETOPROTEIN IN PHYSIOLOGICAL AND PATHOLOGICAL STATE[J].Chinese Journal of Biochemistry and Molecular Biology,1986,2(3):7-12.
Authors:Xu  Kai-Li Zhou  Jin
Institution:(Shanghai Cancer Institute)Wang,Kang-Mei (Cancer Hospital,Shanghai Medical University
Abstract:Molecular heterogeneity patterns of human serum AFP in physiological and pathological state as revealed by lectin-affinity characterization using Concanvalin A (Con A) and Lens Calinaris agglutinin (LCA) as ligand in two dimensional radio-immunoelectrophoresis (Af f-RIEP) were studied.As shown in Con A Af f-RIEP,one AFP variant reactive to Con A was found in serum of patients suffering from acute hepatitis and in normal pregnancy,while two variants,with the major one being able to form Con A bound AFP and the minor one inactive in Con A binding,were found in serum of patients with primary liver cancer.Although two variants of AFP were found in human sera as shown in LCA-Aff-RIEP,the proportion of LCA binding AFP was lower in serum of hepatitis and pregnancy than in serum of primary liver cancer.Since the patterns of AFP variants in adult regeneration of hepatocytes and fetal liver are different from that in the alteration of AFP glycosylation may occur during hepatocarcinogenesis.
Keywords:Alpha-fetoprotein(AFP)  Lectin-Aff-RISP  AFP variants  Molecular heterogeneity of AFP  Physiological and pathological atate  
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