| 大鼠脂多糖结合蛋白的分离纯化及对脂多糖的调节作用 |
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| 引用本文： | 侯一峰,毛宝龄,钱桂生,徐剑铖.大鼠脂多糖结合蛋白的分离纯化及对脂多糖的调节作用[J].中国生物化学与分子生物学报,2001,17(5):647-650. |
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| 作者姓名： | 侯一峰 毛宝龄 钱桂生 徐剑铖 |
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| 作者单位： | 第三军医大学附属新桥医院呼吸病研究所 |
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| 基金项目： | 国家自然科学基金重点资助项目(39730201) |
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| 摘 要： | 经硫酸铵沉淀、Bio Rex70树脂的阳离子交换层析和MonoQ预装柱的阴离子交换层析 ,从大鼠急性期血清中分离纯化了脂多糖结合蛋白 .SDS PAGE为单一条带 ,分子量 60kD ,所纯化的大鼠脂多糖结合蛋白可明显增强脂多糖与单核巨噬细胞的结合 .脂多糖结合蛋白对脂多糖诱导肺泡巨噬细胞中肿瘤坏死因子α(TNF α)mRNA表达的调节作用具有明显的剂量依赖性 .
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| 关 键 词： | 脂多糖结合蛋白 脂多糖 分离纯化 TNF-α |
| 收稿时间： | 2001-10-20 |
| 修稿时间： | 2000年12月22 |
Purification of Lipopolysaccharide-binding Protein from Acute Phase Rat Serum and Its Regulatory Effect on Lipopolysaccharide |
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| HOU Yi\|feng\,MAO Bao\|ling,QIAN Gui\|sheng,XU Jian\|cheng.Purification of Lipopolysaccharide-binding Protein from Acute Phase Rat Serum and Its Regulatory Effect on Lipopolysaccharide[J].Chinese Journal of Biochemistry and Molecular Biology,2001,17(5):647-650. |
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| Authors: | HOU Yi\|feng\ MAO Bao\|ling QIAN Gui\|sheng XU Jian\|cheng |
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| Institution: | (Institute of Respiratory Diseases,Xinqiao Hospital,Third Military Medical University,Chongqing\ 400037,China |
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| Abstract: | The lipopolysaccharide\|binding protein form actue phase rat serum was purified by precipitation of ammonium sulphate,Bio\|Rex70 resin and MonoQ column. SDS\|PAGE analysis indicated that the purified preparation of rat lipopolysaccharide\|binding protein showed homogeneity and had molecular weight of 60 kD.The binding of lipopolysaccharide to mononuclear cells or macrophages were enhanced by purified lipopolysaccharide\|binding protein and the regulatory effect of lipopolysaccharide\|binding protein on TNF\| α mRNA expression induced by lipopolysaccharide in alveolar macrophage was dose dependent. |
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| Keywords: | lipopolysaccharide-binding protein purification lipopolysaccharide TNF\| α |
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