Apelin-13促进血管平滑肌细胞增殖、迁移和血管新生内膜增生

研究apelin-13对血管平滑肌细胞（vascular smooth muscle cell, VSMC）增殖和迁移的影响及其作用机制.用免疫印迹分析检测apelin-13对VSMC增殖、迁移以及分化相关基因表达的影响，结果表明，apelin-13能以时间和浓度依赖的方式诱导VSMC增殖和迁移相关基因cyclin D1和MMP-2表达，促进细胞增殖和迁移；同时使VSMC分化标志基因SM22α和SM α-actin表达水平降低.而且，用鬼笔环肽对细胞骨架进行染色的结果显示，apelin-13可以促进VSMC从收缩表型向增殖表型转化.体内实验也表明，敲低apelin可抑制球囊损伤诱导的新生内膜形成，提示apelin-13在体内具有促进血管新生内膜形成的作用.总之，本文结果表明，apelin 13通过调节VSMC增殖、迁移以及分化基因表达，进而促进其从分化型向增殖型转化，并向内膜下迁移和增殖.

Apelin-13 Promotes Vascular Smooth Muscle Cell Proliferation,Migration and Neointima Hyperplasia

To investigate the effect of apelin-13 on vascular smooth muscle cell (VSMC) phenotypic modulation, the expression of VSMC proliferation , migration- and differentiation-related genes was detected by Western blot analysis. The results showed that apelin-13 increased the expression of proliferation related gene cyclin D1 and migration-related gene MMP-2 in a time- or dose- dependent manner, whereas the expression of differentiation marker genes SM22α and SM α-actin were decreased. Phalloidin staining showed that apelin-13 treatment promoted the transition of VSMCs from the differentiation phenotype to the proliferation phenotype. In vivo experiments demonstrated that the knockdown of apelin-13 inhibited neointimal formation, suggesting its potential role to increase neointima hyperplasia.