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Shear field mapping in actin networks by using magnetic tweezers
Authors:Frank G Schmidt  Florian Ziemann  Erich Sackmann
Institution:(1) Physics Department E22 (Biophysics Group), Technische Universität München, James-Franck-Strasse, D-85747 Garching, Germany;(2) Present address: Institute for Theoretical Physics, University of California, Santa Barbara, California, USA
Abstract:An improved magnetic bead microrheometer based on phase contrast microscopy allowing high resolution measurements of local deformations within macromolecular networks is applied to study local viscoelastic properties of cross-linked actin networks. By embedding non-magnetic colloidal beads as probes into the networks, the spatial variation of the strain field within cross-linked actin networks can be mapped. Moreover, the Poisson ratio and shear modulus can be measured locally.
Keywords:Biogels  actin networks  magnetic tweezers  microrheology of polymer networks  cytoskeleton viscoelasticity
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