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| 利用时间进程法优化活性污泥DG-DGGE图谱 | |
| 引用本文： | 宋业颖,赵丽华,邢德峰,徐香玲,任南琪.利用时间进程法优化活性污泥DG-DGGE图谱[J].生物技术,2006,16(2):43-45. |
| 作者姓名： | 宋业颖 赵丽华 邢德峰 徐香玲 任南琪 |
| 作者单位： | 1. 哈尔滨师范大学生命与环境科学学院,黑龙江,哈尔滨,150080;哈尔滨工业大学市政环境工程学院,黑龙江,哈尔滨,150090 2. 黑龙江大学,生命科学学院,黑龙江,哈尔滨,150080 3. 哈尔滨工业大学市政环境工程学院,黑龙江,哈尔滨,150090 4. 哈尔滨师范大学生命与环境科学学院,黑龙江,哈尔滨,150080 |
| 基金项目： | 国家自然科学基金项目(30470054) |
| 摘 要： | 目的:为了探讨电泳时间对双梯度-变性梯度凝胶电泳(DG-DGGE)分析活性污泥样品时的影响。方法:提取污泥DNA后,以通用引物338f/534r扩增16S rDNA序列,采用时间进程法优化PCR扩增产物的DG-DGGE分离效果。结果:采用不同电泳时间进行DGGE分析时,DGGE图谱存在显著的差异。16S rDNA V3区(200 bp)在凝胶梯度6%~12%,变性剂梯度30%~60%时,在200V电压下,最佳电泳时间为5h。 |
| 关 键 词： | DGGE 16SrDNA 时间进程法 活性污泥 |
| 文章编号： | 1004-311X（2006）02-0043-03 |
| 收稿时间： | 2005-11-03 |
| 修稿时间： | 2005-12-23 |
Optimization of Profiles of Denaturing Gradient Gel Electrophoresis for Activated Sludge by Time Travel |
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| SONG Ye-ying,ZHAO Li-hua,XING De-feng,XU Xiang-ling,REN Nan-qi.Optimization of Profiles of Denaturing Gradient Gel Electrophoresis for Activated Sludge by Time Travel[J].Biotechnology,2006,16(2):43-45. | |
| Authors: | SONG Ye-ying ZHAO Li-hua XING De-feng XU Xiang-ling REN Nan-qi |
| Institution: | 1. College of Life and Environmental Science, Harbin Normal University, Harbin 150080, China;2. College of Life Science, Heilongiiang University, Harbin 150080, China;3. School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China |
| Abstract: | Objectives:In order to discuss effect of electrophoresis times on the analysis of microbial community of activated sludge based on targeted sequence of 16S rDNA by double gradient denaturing gradient gel electrophoresis(DG-DGGE).Methods:Genomic DNA was isolated from activated sludge, and 16S rDNA fragments were amplified with there primer sets(338f/534r).PCR products were separated by DG-DGGE,and optimal electrophoresis time was determined by time travel experiment.Results: The results indicated that DGGE profiles were obviously different under different electrophoresis times.While PCR products of V3 region of 16 rDNA(200 bp) were separated in the gel containing 6%-12% acrylamide and 30%-60% denaturant under 200 V,optimal electrophoresis time was 5 hours. |
| Keywords: | DGGE 16S rDNA time travel activated sludge |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |