| L-缬氨酸产生菌的选育及其代谢流分析 |
| |
| 引用本文： | 李秀敏,杨毅,姜绪林,张星元.L-缬氨酸产生菌的选育及其代谢流分析[J].生物技术,2003,13(6):19-21. |
| |
| 作者姓名： | 李秀敏 杨毅 姜绪林 张星元 |
| |
| 作者单位： | 江南大学工业生物技术教育部重点实验室,江苏,无锡,214036 |
| |
| 摘 要： | 以谷氨酸棒杆菌(Corynebacterium glutamicum)AS1.495(Leu^-)为出发菌株，通过多次亚硝基胍(NTG)诱变，给AS1．495(Leu^-)依次叠加L-AAH^as，2-TA^r，Vd^-的遗传标记，得到突变株AATV341(Leu^-，L-AAH^as，2-TA^r，Vd^-)，可在8％的葡萄糖培养基积累L-缬氨酸24．5g／L，比出发菌株提高了5．13倍。同时运用代谢流量分析理论，测定出发菌株AS1．495及其突变株AATV341在L-缬氨酸合成阶段的代谢流量，并初步进行比较和分析，发现遗传标记的引入使流量分配发生了重大变化，流量分配朝着有利于L-缬氨酸合成的方向改变。
|
| 关 键 词： | L-缬氨酸 产生菌 选育 代谢流分析 谷氨酸棒杆菌 亚硝基胍诱变 |
| 文章编号： | 1004-311X(2003)06-0019-03 |
| 修稿时间： | 2003年8月4日 |
Breeding of L-valine Producer and Its Metabolic Flux Analysis |
| |
| LI Xiu-min,YANG Yi,JIANG Xu-lin,ZHANG Xing-yuan.Breeding of L-valine Producer and Its Metabolic Flux Analysis[J].Biotechnology,2003,13(6):19-21. |
| |
| Authors: | LI Xiu-min YANG Yi JIANG Xu-lin ZHANG Xing-yuan |
| |
| Abstract: | AATV341(Leu~-,L-AAH~(ss),2-TA~r,Vd~-) was obtained by N-methyl-N'-nitro-N-nitroso-guanidine (NTG) mutagenesis derived from the original strain Corynebacterium glutamicum AS1.495(Leu~-).AATV341 can produce 25.4 g/L of L-valine in 8% glucose medium,which increased 5.13 times more than AS1.495.According to the theory of metabolic flux analysis,the metabolic flux of Corynebacterium glutamicum AS1.495 and its mutants AATV341 was determined,compared and analyzed during L-valine synthesis.The metabolic flux distribution of AS1.495 changed greatly,which was caused by the introduction of genetic markers and was useful to L-valine synthesis. |
| |
| Keywords: | L-valine Corynebacterium glutamicum NTG mutagenesis Breeding Metabolic flux analysis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
