| 玉米抗丝黑穗病基因RAPD分析 |
| |
| 引用本文： | 李玥莹,董雪卉,许丽,陶思源.玉米抗丝黑穗病基因RAPD分析[J].生物技术,2006,16(5):16-18. |
| |
| 作者姓名： | 李玥莹 董雪卉 许丽 陶思源 |
| |
| 作者单位： | 1. 沈阳师范大学化学与生命科学学院,辽宁,沈阳,110034
2. 沈阳农业大学,辽宁,沈阳,110161 |
| |
| 基金项目： | 沈阳市科技计划项目(1041053-6-04)，沈阳师范大学实验中心主任基金项目资助 |
| |
| 摘 要： | 该文应用RAPD技术,以感病母本3045和抗病父本3024为试材,应用BSA法,对玉米抗丝黑穗病基因进行分析,建立并优化了玉米的RAPD反应体系,筛选了61条引物RAPD随机引物,18条引物为适宜引物,共扩增出89条DNA谱带,得到了4条具有稳定多态性标记的引物,分别为OPY-09、OPY-10、OPY-18、OPP-09。
|
| 关 键 词： | 玉米 丝黑穗病 RAPD |
| 文章编号： | 1004-311X（2006）05-0016-03 |
| 收稿时间： | 2006-05-08 |
| 修稿时间： | 2006-09-14 |
RAPD Analysis of Head Smut Resistance Gene on Maize |
| |
| LI Yue-ying,DONG Xue-hui,XU Li,TAO Si-yuan.RAPD Analysis of Head Smut Resistance Gene on Maize[J].Biotechnology,2006,16(5):16-18. |
| |
| Authors: | LI Yue-ying DONG Xue-hui XU Li TAO Si-yuan |
| |
| Institution: | 1. Collage of Chemical and Biological Science, Shenyang Normal University Shenyang 11034;2.Shenyang Agriculture Univemity, Shenyang 110161, Oaina |
| |
| Abstract: | With Bulked Segregation Analysis (BSA),61 random sequence dicer-primer were used to screen a pair of bulked samples for markers linked to the head smut resistance gene of maize.RAPD reaction system in maize was established and optimaized.Under this experiment condition, 18 random sequence dicer-primer were high efficient.89 bands of DNA were amplified.Polymorphism bands were amplified with primer OPY- 09,OPY-100,OPY-18,OPP-09. |
| |
| Keywords: | maize head smut RAPD |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
